Proliferation of the murine corticotropic tumour cell line AtT20 is affected by hypophysiotrophic hormones, growth factors and glucocorticoids

Molecular and Cellular Endocrinology
P A van WijkJ A Mol

Abstract

In pituitary-dependent hyperadrenocorticism (Cushing's disease), the disturbed regulation of ACTH secretion is associated with neoplastic transformation of corticotropic cells. As these two phenomena are almost indissolubly connected, it is of prime importance to elucidate the factor(s) that induce corticotropic cell proliferation. Here we report on the effects of hypophysiotrophic hormones and intrapituitary growth factors on the proliferation and hormone secretion of the murine corticotropic tumour cell line AtT20/D16v, as measured by DNA content, and ACTH concentration in culture media. In addition, sensitivity to the inhibitory effect of cortisol was assessed under various conditions. Corticotropin releasing hormone (CRH) and vasopressin (AVP) induced proliferation of AtT20-cells. In contrast to that caused by AVP, the CRH-induced proliferation was associated with increased ACTH secretion, which could be inhibited by cortisol. Insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) also stimulated the proliferation of AtT20-cells. The proliferation of AtT20-cells was significantly inhibited by cortisol in all tests. The IGF-I-induced proliferation was the least sensitive...Continue Reading

References

Jul 1, 1990·The Journal of Clinical Investigation·J M AlexanderA Klibanski
Dec 1, 1990·The Journal of Clinical Endocrinology and Metabolism·V HermanS Melmed
Mar 1, 1990·Proceedings of the Society for Experimental Biology and Medicine·S L AsaW Vale
Aug 1, 1990·The Journal of Endocrinology·A M McNicolW McMeekin
May 1, 1987·Endocrine Reviews·D GospodarowiczG Neufeld
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N FerraraD Gospodarowicz
Oct 1, 1987·Molecular and Cellular Endocrinology·B Lutz-BucherB Koch
Feb 1, 1988·The Journal of Clinical Endocrinology and Metabolism·A LugerG P Chrousos
Feb 1, 1988·Endocrine Reviews·J R Lundblad, J L Roberts
Apr 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A G KasselbergM T Stahlman
Aug 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A BairdR Guillemin
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Aug 1, 1994·Molecular and Cellular Endocrinology·A G SchullerS L Drop

❮ Previous
Next ❯

Citations

Sep 1, 2005·Pituitary·Guillaume AssiéXavier Bertagna
Nov 15, 2002·Molecular and Cellular Endocrinology·Anna Spada, Andrea Lania
May 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Li DuAnthony P Heaney
Apr 25, 2000·In Vitro Cellular & Developmental Biology. Animal·A T SlominskiJ Wortsman
Sep 16, 2011·Endocrine-related Cancer·Odelia CooperShlomo Melmed
Jul 3, 2013·The Journal of Endocrinology·Erica GentilinMaria Chiara Zatelli
Jul 30, 2010·European Journal of Endocrinology·T M BarberJ A H Wass
Jul 10, 2010·European Journal of Endocrinology·Yuji TaniYukio Hirata
Nov 26, 2013·Endocrine·Diego FeroneRosario Pivonello
Oct 23, 2008·Regulatory Peptides·Kazunori KageyamaToshihiro Suda
Apr 16, 2016·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Haruo NogamiSadakazu Aiso
Apr 30, 2011·Molecular Endocrinology·Yvonne BockmühlDietmar Spengler
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A SlominskiE T Wei
Apr 7, 2007·Endocrinology·Sivan Subburaju, Greti Aguilera
Mar 29, 2011·Stress : the International Journal on the Biology of Stress·Kazunori KageyamaToshihiro Suda

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.