Proliferation of Toxoplasma gondii in inflammatory macrophages in vivo is associated with diminished oxygen radical production in the host cell

International Journal for Parasitology
Sunder P ShresthaAmos Orlofsky

Abstract

While reactive oxygen species (ROS) can kill Toxoplasma gondii in vitro the role these molecules play in vivo is not known. We used a flow cytometry-based assay to investigate the relationship between intracellular infection and ROS production during acute peritoneal toxoplasmosis in mice. A distinct population of ROS(+) inflammatory macrophages, detected by the oxidation of hydroethidine, was observed to increase progressively in frequency during the course of infection, and to be inversely correlated with the degree of cell parasitization. These data imply that either intracellular parasites inhibit ROS synthesis or, alternatively, ROS-producing cells contain anti-Toxoplasma activity. The latter interpretation was supported by the finding that uninfected ROS-producing inflammatory macrophages were resistant to infection in vivo. However, in the same animals, ROS-producing macrophages that had previously been parasitized could readily be infected with additional parasites, suggesting that the difference in ROS production between highly infected and less infected cells was not due to ROS-associated killing of parasites within these cells. In addition, macrophages infected with T. gondii in vitro and then briefly transferred to ...Continue Reading

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Citations

Nov 26, 2008·The Journal of Biological Chemistry·Yubao WangAmos Orlofsky
Mar 31, 2011·International Journal for Parasitology·Benjamin MorgaIsabelle Arzul
Jul 4, 2020·The Korean Journal of Parasitology·Hei Gwon ChoiGuang-Ho Cha
Oct 24, 2017·Frontiers in Cellular and Infection Microbiology·Benjamin MorgaIsabelle Arzul
May 23, 2019·Frontiers in Immunology·Marta García-SánchezEsther Collantes-Fernández
Jun 12, 2020·Frontiers in Cellular and Infection Microbiology·Caroline M MotaTiago W P Mineo
Oct 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tadakimi TomitaAmos Orlofsky
Mar 23, 2017·Frontiers in Immunology·Murilo Vieira da SilvaTiago Wilson Patriarca Mineo
May 2, 2019·Frontiers in Cellular and Infection Microbiology·Tatiane S Lima, Melissa B Lodoen
Mar 7, 2021·Antioxidants·Marc Herb, Michael Schramm
Aug 28, 2021·Frontiers in Cellular and Infection Microbiology·Eduardo AlvesMatthew A Child

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