PMID: 3321379Jan 1, 1987Paper

Proliferative activity of cells in the synovium as demonstrated by a monoclonal antibody, Ki67

Rheumatology International
P A LalorP A Revell

Abstract

The presence of proliferating cells has been sought in the synovium of rheumatoid arthritic (RA) and osteoarthritic (OA) joints using the monoclonal antibody Ki67, which marks a nuclear antigen present in all stages of the cell cycle apart from Go. Synovia were studied from 21 RA and 14 OA cases using the indirect immunoperoxidase technique. Double-staining was performed on 18 RA and 17 OA synovia with the simultaneous labelling of lysozyme (muramidase) by the immuno-alkaline phosphatase method and with Ki67 by the indirect immunoperoxidase method. Most of the RA and OA synovia showed an absence of Ki67-positive cells in the intimal cell layer. Three RA and four OA synovia showed no more than ten proliferating cells in the whole of the intimal layer examined. Similar results were obtained when double-labelling was performed. Eight RA and six OA synovia showed the presence of occasional Ki67-positive cells in the intimal layer. The total number of intimal cells was measured for each histological section, and the proliferation index calculated as the percentage of total cells in the intimal layer showing Ki67-positive staining. This varied between 0.03% and 0.0033% (between 1:2800 and 1:30,000 cells). In contrast, there were plen...Continue Reading

References

Jun 1, 1975·Annals of the Rheumatic Diseases·W MohrW Mohing
Jan 1, 1987·Annals of the Rheumatic Diseases·P I Mapp, P A Revell
May 1, 1972·Annals of the Rheumatic Diseases·A L Macafee
Sep 1, 1972·Annals of the Rheumatic Diseases·L E Glynn
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·J GerdesH Stein
Feb 1, 1983·Scandinavian Journal of Dental Research·S KoppE Clemensson
Feb 1, 1983·Journal of the American Podiatry Association·B S Schlefman, F N Katz
Jun 1, 1980·Annals of the Rheumatic Diseases·L A CoultonJ Chayen
Aug 1, 1962·The Journal of Cell Biology·P BARLANDD HAMERMAN

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Citations

Jan 1, 1989·Rheumatology International·P A Revell
Jan 1, 1988·Rheumatology International·P I Mapp, P A Revell
Apr 1, 1992·DNA and Cell Biology·G BandaraC H Evans
Jan 19, 2012·Rheumatology·Valérie VilmontGilles Chiocchia
Dec 1, 1990·Histopathology·D C Brown, K C Gatter
Apr 1, 1988·Annals of the Rheumatic Diseases·B HendersonJ C Edwards
Jan 1, 1994·Annals of the Rheumatic Diseases·R MorrisC J Morris
Apr 1, 1994·Annals of the Rheumatic Diseases·P I MappD R Blake
May 1, 1995·Annals of the Rheumatic Diseases·N A Athanasou
Jun 1, 1995·Annals of the Rheumatic Diseases·R W KinneF Emmrich
Jun 1, 1995·Annals of the Rheumatic Diseases·O FitzGerald, B Bresnihan
Mar 1, 1990·Journal of Clinical Pathology·P A Hall, D A Levison
Jan 20, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maxim A MorozRonald G Blasberg
Nov 30, 2000·Arthritis Research·P P Tak, F C Breedveld
Mar 17, 2011·PloS One·Alessandra RigamontiChad A Cowan
Jan 1, 1988·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·N BraunH K Müller-Hermelink
Feb 9, 2005·The Journal of Cell Biology·Dongren YangBruce L Patton
Jan 1, 1995·Scandinavian Journal of Rheumatology. Supplement·N J Zvaifler
Jan 1, 1988·Scandinavian Journal of Rheumatology. Supplement·B HelbigH K Müller-Hermelink
Jan 26, 2016·Biochemical and Biophysical Research Communications·Yusuke MatsuoHitoshi Kohsaka
Jan 22, 2002·International Journal of Cancer. Journal International Du Cancer·Sofia Thelin-JärnumPierre Aman
Oct 1, 2004·Arthritis and Rheumatism·Guillermo PalaoJosé L Pablos
May 1, 2009·Arthritis and Rheumatism·John A Hamilton, Paul P Tak
Dec 20, 2007·The European Journal of Neuroscience·Perrine BarraudRobin J M Franklin

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