Proliferative activity of skeletal myoblast sheet by paracrine effects of mesenchymal stem cells

Journal of Oral Biosciences
Takashi UmezawaShinichi Abe

Abstract

The purpose of this study was to examine the proliferative activity enhancement of skeletal myoblasts in the presence and absence of mesenchymal stem cells (MSC). We artificially fabricated two types of cell sheets by co-culturing rabbit skeletal myoblast sheets with rabbit MSCs using type IA collagen gel (MC+), and cell sheets without rabbit MSCs (MC-). The results of hematoxylin and eosin staining revealed that MC+ was thicker than MC- on day 7. Immunohistochemical staining revealed a low level of desmin expression in both sheets on day 2. Desmin expression increased at days 7 and 12, and desmin localization was consistent with the stratified area. Reverse transcription PCR revealed the presence of MyoD and PAX7 in both sheets on days 2, 7, and 12. The presence of myogenin was confirmed in both sheets on days 7 and 12. Hepatocyte growth factor expression was evident in MC+ on day 2, and in both sheets on day 7. Measurement of cell proliferative activity based on DNA cell cycle analysis indicated that MC+ had significantly higher cell proliferative activity than MC- on day 7. There were no significant differences in cell proliferative activity between MC+ and MC- on day 12. This study demonstrated that the presence of MSCs cou...Continue Reading

Citations

Jan 10, 2020·Anatomical Science International·Masahito Yamamoto, Shinichi Abe
Jan 6, 2018·Npj Regenerative Medicine·Kazuhisa YamamotoHiromi Kojima
Jul 3, 2018·The Bulletin of Tokyo Dental College·Toshihito YoshimotoShinichi Abe

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