PMID: 6403454Jan 1, 1983Paper

Proliferative kinetics and mitomycin C-induced chromosome damage in Fanconi's anemia lymphocytes

Human Genetics
K MiuraA Koizumi

Abstract

Lymphocytes from two sisters with Fanconi's anemia (FA) were studied for cell cycle kinetics, sister chromatid exchanges (SCEs), and chromosomal aberrations when they had undergone one, two, or three or more divisions in mitomycin C (MMC)-treated cultures. Lymphocytes from the parents, another sister of the probands, and a healthy unrelated adult were examined as controls. Analyses of cell cycle kinetics by the sister chromatid differential staining method revealed that the relative frequency of metaphase cells at their third or subsequent divisions was much smaller in untreated FA cultures than in normal cultures fixed at 96 h after phytohemagglutinin stimulation. These data indicate that FA cells proliferate much more slowly than normal cells. MMC treatments of FA and normal cells led to a clearly dose-related delay in cell turnover times, the duration of delay being much longer in FA than in normal cells. FA cells had about 1.4 times higher frequencies of SCEs than normal cells in both MMC-treated and untreated cultures. FA cells also showed several times higher frequencies of chromosomal aberrations than normal cells, and the frequency of chromosomal aberrations decreased through subsequent mitoses by approximately 60% in b...Continue Reading

References

Nov 1, 1979·Journal of Cellular Physiology·R WeksbergL Siminovitch
Nov 1, 1979·Mutation Research·S B BigelowM A Bender
Nov 24, 1978·Human Genetics·M Hirsch-KauffmannK Sperling
Jul 15, 1977·Journal of Molecular Biology·Y Fujiwara, M Tatsumi
Jun 29, 1976·Human Genetics·T M SchroederF Vogel
Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·S A LattP S Gerald
Dec 1, 1975·Journal of Cellular Physiology·E Elmore, M Swift
Feb 1, 1973·Journal of Theoretical Biology·A V Carrano, J A Heddle
Sep 1, 1971·Blood·M Higurashi, P E Conen
Apr 1, 1965·Vision Research·H J Dartnall, J N Lythgoe
Jan 6, 1966·The New England Journal of Medicine·G E BloomL K Diamond
Sep 1, 1966·Annals of Internal Medicine·M R Swift, K Hirschhorn
Apr 29, 1967·Nature·M S Sasaki, A Norman
Dec 11, 1980·Nature·K Morimoto, S Wolff

❮ Previous
Next ❯

Citations

Aug 1, 1993·Cancer Genetics and Cytogenetics·R BergerM C Gendron
Dec 1, 1985·Mutation Research·B PorfirioE Gandini
Jan 1, 1987·Journal of Environmental Science and Health. Part A, Environmental Science and Engineering·Merlin G ButlerBonnie B Jenkins

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.