PMID: 7542296Jul 1, 1995Paper

Proliferative potential of different keratinocytes of plucked human hair follicles

The Journal of Investigative Dermatology
I Moll

Abstract

We have examined colony-forming ability, localization of colony-forming cells, and in vitro life spans of outer root sheath keratinocytes of different fragments of adult human plucked hair follicles. These were shown by immunohistochemical staining for cytokeratins and integrins to contain a preserved basal cell layer. By microdissection, five fragments of the outer root sheath (B1, B2, B3-1, B3-2, B4) were separated, dispersed by trypsin into single cell suspensions, and grown on human feeder fibroblasts. All fragments gave rise to at least some colonies, but colony-forming ability was mostly marked in the intermediate part (B2) and the lower half of the central part (B3-1); approximately 60% of colony-forming cells of a hair follicle localized to the fragment B3-1 and 28% to the fragment B3-2 (upper half of the central part, including bulge). To compare the in vitro life spans of cells from the various fragments, we subcultured isolated keratinocytes under identical conditions. The longest was found in the fragment B3-2 and the shortest in the fragment B1 (bulb). Moreover, the differentiation state of the native cells and the cells of all cultures were studied during their whole life spans by immunocytochemical analysis of va...Continue Reading

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Citations

May 20, 2004·The British Journal of Dermatology·C G GhoF C S Ramaekers
Apr 11, 2007·The Journal of Cell Biology·René VilladsenOle W Petersen
Aug 26, 2018·Scientific Reports·Michaela Zorn-KruppaJohanna M Brandner
Sep 7, 1999·In Vitro Cellular & Developmental Biology. Animal·M MichelL Germain
Mar 22, 2006·Pediatric Research·Cecilia Roh, Stephen Lyle
Dec 8, 2006·Archives of Dermatological Research·Justyna Niderla-BielińskaStanisław Moskalewski
Sep 3, 2011·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Megumi TakikawaTomoharu Kiyosawa
Sep 18, 2007·Stem Cell Reviews·Mark A LaBargeMina J Bissell
Oct 20, 2006·Plastic and Reconstructive Surgery·Carlos Oscar UebelPedro Martins
Jul 24, 2008·Archives of Dermatological Research·Justyna Niderla-BielinskaStanislaw Moskalewski
Dec 5, 2013·TheScientificWorldJournal·Kevin SchembriDuncan Ayers
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·G I TopleyG P Dotto
Mar 17, 2012·The Journal of Craniofacial Surgery·Tong HanYa Qin Zhang
May 27, 2010·Experimental Dermatology·Araika Gutiérrez-RiveraAnder Izeta
Jan 17, 2021·Stem Cell Research & Therapy·Carla M AbreuAlexandra P Marques
Apr 14, 1999·The Journal of Investigative Dermatology·R J Morris, C S Potten
Jan 29, 2000·The Journal of Investigative Dermatology·M AkiyamaH Shimizu
Feb 13, 2003·Journal of the American Academy of Dermatology·Alain LimatDenis Salomon
Feb 1, 1997·The Journal of Investigative Dermatology·I MollW W Franke
Nov 12, 1998·Differentiation; Research in Biological Diversity·L Weiner, H Green
Dec 16, 2005·The Journal of Investigative Dermatology·Cecilia RohStephen Lyle
Feb 7, 2006·BMC Biology·Jung Yeon KimDarryl Shibata

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