Proliferative response of human CD4+ T lymphocytes stimulated by the lectin jacalin

European Journal of Immunology
E BlascoJ L Preud'homme

Abstract

The Gal beta(1-3)GalNAc-binding lectin jacalin is known to specifically induce the proliferation of human CD4+ T lymphocytes in the presence of autologous monocytes and to interact with the CD4 molecule and block HIV-1 infection of CD4+ cells. We further show that jacalin-induced proliferation is characterized by an unusual pattern of T cell activation and cytokine production by human peripheral blood mononuclear cells (PBMC). A cognate interaction between T cells and monocytes was critical for jacalin-induced proliferation, and human recombinant interleukin (IL)-1 and IL-6 did not replace the co-stimulatory activity of monocytes. Blocking studies using monoclonal antibodies (mAb) point out the possible importance of two molecular pathways of interaction, the CD2/LFA-3 and LFA-1/ICAM-1 pathways. One out of two anti-CD4 mAb abolished jacalin responsiveness. Jacalin induced interferon-gamma and high IL-6 secretion, mostly by monocytes, and no detectable IL-2 synthesis or secretion by PBMC. In contrast, jacalin-stimulated Jurkat T cells secreted IL-2. CD3- Jurkat cell variants failed to secrete IL-2, suggesting the involvement of the T cell receptor/CD3 complex pathway in jacalin signaling. IL-2 secretion by CD4- Jurkat variant ce...Continue Reading

References

Oct 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K E BurgessC E Rudd
Feb 5, 1992·Journal of Immunological Methods·J L Preud'hommeN Gualde
Jan 1, 1991·Molecular Immunology·N PineauK Kobayashi
Jul 5, 1991·Journal of Immunological Methods·I K de Miranda-SantosA Campos-Neto
Jun 1, 1990·Clinical and Experimental Immunology·N PineauJ L Preud'homme
Feb 9, 1990·Journal of Immunological Methods·J S PeacockV B Pinto
Oct 1, 1985·The Journal of Experimental Medicine·I Bank, L Chess
Jan 1, 1989·Journal of Clinical Laboratory Analysis·P AucouturierJ L Preud'homme
Mar 1, 1988·The Journal of Experimental Medicine·M LotzD A Carson
Oct 1, 1988·Molecular Immunology·K KobayashiJ P Vaerman
Feb 1, 1988·Immunology Letters·C R GattassM M Bunn-Moreno
May 1, 1987·Molecular Immunology·P AucouturierJ L Preud'homme

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Citations

Apr 3, 2001·Molecular Immunology·J L Preud'hommeE Lelièvre
Apr 13, 2002·Annals of the New York Academy of Sciences·Grant R YeamanGillian A Lang
Sep 11, 2009·Journal of Photochemistry and Photobiology. B, Biology·Gunjan PandeySneha Sudha Komath
Nov 24, 2004·Protein Science : a Publication of the Protein Society·Anagh A SahasrabuddheM Islam Khan
Apr 28, 2016·BioMed Research International·Cláudia Danella PolliGabriela Pereira-da-Silva
Dec 12, 2012·Critical Reviews in Microbiology·Ram Sarup Singh, Amandeep K Walia
Sep 4, 1999·Parasite Immunology·D I PritchardE Beck
Jun 8, 2018·Life Sciences·Elba V M M CarvalhoMaria T S Correia
Oct 29, 2021·Applied Biochemistry and Biotechnology·V LavanyaShazia Jamal

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