Proliferative response of the stem cell system during regeneration of the rostrum in Macrostomum lignano (Platyhelminthes)

Cell and Tissue Research
Freija VerdoodtMaxime Willems

Abstract

Macrostomum lignano (Platyhelminthes) possesses pluripotent stem cells, also called neoblasts, which power its extraordinary regeneration capacity. We have examined the cellular dynamics of neoblasts during regeneration of the rostrum in M. lignano. First, using live squeeze observations, the growth curve of the rostrum was determined. Second, neoblasts were labelled with 5-bromo-2'-deoxyuridine (BrdU) and an anti-phospho-histone H3 mitosis marker (anti-phos-H3) to analyze their proliferative response to amputation. During the regeneration process, both S- and M-phase cells were present anterior to the eyes, a region that is devoid of proliferating cells during homeostasis. Furthermore, BrdU pulse experiments revealed a biphasic S-phase pattern, different from the pattern known to occur during regeneration of the tail plate in M. lignano. During a first systemic phase, S-phase numbers significantly increased, both in the region adjacent to the wound (the anterior segment) and the region far from the wound (the posterior segment). During the second, spatially restricted phase, S-phase numbers in the anterior segment rose to a peak at 3 to 5 days post-amputation (p-a), while in the posterior segment, S-phase activity approached c...Continue Reading

References

Nov 21, 1998·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·A Sánchez Alvarado, P A Newmark
Feb 7, 2001·Developmental Biology·P A Newmark, A Sánchez Alvarado
Jul 13, 2002·The Journal of Experimental Zoology·Emili Saló, Jaume Baguñà
Jun 14, 2003·Proceedings. Biological Sciences·Lukas Schärer, Peter Ladurner
Apr 1, 1962·Canadian Journal of Microbiology·R R GUILLARD, J H RYTHER
Oct 11, 2003·Current Opinion in Genetics & Development·Kiyokazu Agata
Apr 27, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Katharina T NimethPeter Ladurner
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Peter W Reddien, Alejandro Sánchez Alvarado
Apr 15, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Emili Saló
Oct 18, 2006·Cell and Tissue Research·Katharina Theresia NimethRobert Gschwentner
Dec 30, 2008·Experimental Gerontology·Stijn MoutonGaetan Borgonie
Feb 4, 2010·Cell and Tissue Research·Katrien De MulderPeter Ladurner
Jul 6, 2010·Developmental Biology·Danielle Wenemoser, Peter W Reddien

❮ Previous
Next ❯

Citations

Nov 30, 2012·Journal of Tissue Engineering and Regenerative Medicine·Silke SchwarzNicole Rotter
Jun 4, 2016·BMC Developmental Biology·Birgit LengererPeter Ladurner
May 3, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·Lorenzo Ricci, Mansi Srivastava
Mar 12, 2020·EvoDevo·Jakub WudarskiEugene Berezikov

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.