PMID: 7519915Jan 1, 1994Paper

Proliferative responses of peripheral blood mononuclear cells from patients with Graves' disease to synthetic peptides epitopes of human thyrotropin receptor

Thyroid : Official Journal of the American Thyroid Association
Y OkamotoL J DeGroot

Abstract

In Graves' disease thyrotropin receptor (TSH-R) autoantibodies cause hyperthyroidism. Production of TSH-R autoantibodies must be controlled by specific T cells. In this study we investigated T cell responses to 33 peptides corresponding to the sequence of the extracellular domain of human TSH-R. Peripheral blood mononuclear cells (PBMC) from 12 patients with Graves' disease and 9 healthy subjects were cultured with peptides for 3 days. The proliferative responses of PBMC were analyzed by measurement of [3H]thymidine incorporation. A stimulation index (SI; mean cpm in the presence of peptide/mean cpm in culture medium alone) of more than 3 was considered a positive response. When PBMC were stimulated with a pool containing all synthesized peptides, the mean SI of patients was significantly higher than that of controls (4.50 +/- 3.95 vs. 1.44 +/- 0.60; p < 0.05). When PBMC were cultured with individual peptides, PBMC from patients responded predominantly to two peptides, corresponding to sequence segments 152-157 (5 patients) and 207-222 (4 patients). No PBMC from controls responded to these two peptides. There was no clear correlation between the HLA-DR or HLA-DQ genotype and the stimulatory sequence segments. These results sugg...Continue Reading

References

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Citations

Dec 24, 1997·Clinical Immunology and Immunopathology·M E FisfalenL J DeGroot
Apr 9, 1998·Baillière's Clinical Endocrinology and Metabolism·A E Heufelder
May 29, 2002·Clinical Endocrinology·E YungG W K Wong
Dec 17, 2002·Thyroid : Official Journal of the American Thyroid Association·Pavel PichurinSandra M McLachlan
Feb 1, 1995·Thyroid : Official Journal of the American Thyroid Association·Y OkamotoL J DeGroot
Feb 1, 1996·Thyroid : Official Journal of the American Thyroid Association·T Yanagawa, L J DeGroot
Feb 12, 1998·Thyroid : Official Journal of the American Thyroid Association·T YanagawaT Saruta
Apr 17, 1998·Thyroid : Official Journal of the American Thyroid Association·S LeeL J DeGroot
Jun 6, 2013·Clinical Endocrinology·Sarah Côté-BigrasDiane Rottembourg
Oct 1, 1995·Thyroid : Official Journal of the American Thyroid Association·T YanagawaL J DeGroot
Apr 14, 2005·Endocrine Reviews·Sandra M McLachlanBasil Rapoport
Dec 23, 1998·Endocrine Reviews·B RapoportS M McLachlan
Nov 14, 1997·The Journal of Clinical Endocrinology and Metabolism·M E FisfalenL J DeGroot
Dec 30, 1999·International Reviews of Immunology·A MartinT F Davies

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