Proline accumulation protects Saccharomyces cerevisiae cells in stationary phase from ethanol stress by reducing reactive oxygen species levels

Yeast
Hiroshi TakagiTomohiro Kaino

Abstract

During fermentation processes, Saccharomyces cerevisiae cells are exposed to multiple stresses, including a high concentration of ethanol that represents toxicity through intracellular reactive oxygen species (ROS) generation. We previously reported that proline protected yeast cells from damage caused by various stresses, such as freezing and ethanol. As an anti-oxidant, proline is suggested to scavenge intracellular ROS. In this study, we examined the role of intracellular proline during ethanol treatment in S. cerevisiae strains that accumulate different concentrations of proline. When cultured in YPD medium, there was a significant accumulation of proline in the put1 mutant strain, which is deficient in proline oxidase, in the stationary phase. Expression of the mutant PRO1 gene, which encodes the γ-glutamyl kinase variant (Asp154Asn or Ile150Thr) with desensitization to feedback inhibition by proline in the put1 mutant strain, showed a prominent increase in proline content as compared with that of the wild-type strain. The oxidation level was clearly increased in wild-type cells after exposure to ethanol, indicating that the generation of ROS occurred. Interestingly, proline accumulation significantly reduces the ROS level...Continue Reading

References

Jul 1, 1986·Molecular and Cellular Biology·S S Wang, M C Brandriss
Mar 15, 2000·Protein Science : a Publication of the Protein Society·D SamuelC Yu
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Changbin Chen, Martin B Dickman
Oct 20, 2005·Journal of Bioscience and Bioengineering·Yuko MoritaHiroshi Takagi
Dec 8, 2005·Applied and Environmental Microbiology·Hiroshi TakagiYoshito Kubo
Dec 31, 2005·Journal of Bioscience and Bioengineering·Keisuke Matsuura, Hiroshi Takagi
Aug 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·Zoya Ignatova, Lila M Gierasch
Mar 28, 2007·Applied Microbiology and Biotechnology·Xiaoyi Du, Hiroshi Takagi
May 16, 2007·Journal of Bioscience and Bioengineering·Hiroshi TakagiYoshito Kubo
Jul 25, 2007·FEMS Microbiology Reviews·Brian R GibsonKatherine A Smart
Nov 27, 2007·Free Radical Biology & Medicine·Navasona KrishnanDonald F Becker
Jul 22, 2008·Applied and Environmental Microbiology·Tomohiro KainoHiroshi Takagi
Sep 13, 2008·Archives of Environmental Contamination and Toxicology·Feijuan WangCheng Zhu
Jan 27, 2009·Biotechnology and Bioengineering·Kaoru IinoyaHiroshi Takagi
May 14, 2010·Applied Microbiology and Biotechnology·Menggen Ma, Z Lewis Liu
Nov 2, 2011·International Journal of Food Microbiology·Yu SasanoHiroshi Takagi
Sep 7, 2012·Plant Signaling & Behavior·Shamsul HayatAqil Ahmad
Jun 19, 2013·Journal of Bioscience and Bioengineering·Yuki Tatehashi, Hiroshi Takagi

❮ Previous
Next ❯

Citations

Oct 22, 2016·International Journal of Food Microbiology·Ariunzaya TsolmonbaatarHiroshi Takagi
Oct 21, 2016·Applied Microbiology and Biotechnology·Rika Indri AstutiHiroshi Takagi
Mar 13, 2018·The Journal of General and Applied Microbiology·Hao JiBin Zhuge
May 16, 2020·Frontiers in Genetics·Noreen Suliani Binti Mat Nanyan, Hiroshi Takagi
Apr 1, 2018·Applied Microbiology and Biotechnology·Ivanna Karina Olivares-MarinCarlos Regalado-Gonzalez
Apr 10, 2019·Current Genetics·Lei QiDao-Qiong Zheng
Apr 7, 2020·Journal of Environmental Management·Rafał SzewczykPrzemysław Bernat
Mar 2, 2021·Applied Microbiology and Biotechnology·Xianlin HeHao Li
Mar 21, 2021·Applied and Environmental Microbiology·Taylor ReiterBen Montpetit
Jun 26, 2021·Bioscience, Biotechnology, and Biochemistry·Yuki YoshikawaHiroshi Takagi
Jul 20, 2021·Synthetic and Systems Biotechnology·Liang XiangJunbiao Dai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.