PMID: 8613891Apr 1, 1996Paper

Proline is synthesized from glutamate during intragastric infusion but not during intravenous infusion in neonatal piglets

The Journal of Nutrition
J M MurphyR O Ball

Abstract

Glutamate is considered the primary precursor amino acid for proline synthesis in mammals. Evidence exists, however, suggesting that proline may be a dietary indispensable amino acid for 2.5-kg piglets due to inadequate synthesis. This hypothesis was tested by intravenous and intragastric infusion of radiolabeled amino acids in vivo. Piglets (3 to 4 d old) were surgically implanted with catheters in the femoral (infusion) and jugular (sampling) veins and in the stomach (feeding and infusion). Piglets were fed hourly, via the stomach catheter, a semi-purified diet containing 10% dried skim milk, 15% corn oil, amino acids, vitamins and minerals. Experiment 1 was a 2 X 2 factorial design, with 24 piglets adapted to either low or supplemental proline diets (1.3 and 16.4 g proline x kg(-1) respectively) for 7 d, then intravenously infused with either [U-14C]glutamate or [U-14C]proline (185 k Bq x kg(-1) prime; 370 kBq x kg(-1) x h(-1) constant) for 4 h. Experiment 2 followed similar protocols, with eight piglets adapted to the low proline diet for 7 d and [U-14C]glutamate or [U-14C]proline infused into the stomach catheter. Piglets infused intravenously with [U-14C]glutamate did not convert glutamate to proline. Radioactive label wa...Continue Reading

Citations

Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·J B van GoudoeverP J Reeds
Jan 20, 2005·American Journal of Physiology. Endocrinology and Metabolism·Kristine L UrschelRonald O Ball
Apr 4, 2006·Journal of Animal Science·B Stoll, D G Burrin
Sep 22, 2010·Pediatric Research·Chris TomlinsonPaul Pencharz
Aug 13, 1999·The American Journal of Physiology·J A BruntonR O Ball
Feb 15, 2001·American Journal of Physiology. Endocrinology and Metabolism·Y M YuV R Young
Jan 16, 2003·American Journal of Physiology. Endocrinology and Metabolism·Robert F P BertoloRonald O Ball
May 20, 2004·American Journal of Physiology. Endocrinology and Metabolism·Dana Lee WilkinsonRonald O Ball
Jun 15, 2006·The Journal of Nutrition·Kristine L UrschelRonald O Ball
Nov 2, 2012·American Journal of Physiology. Endocrinology and Metabolism·Hidehiro NakamuraRyosei Sakai
Jun 25, 2014·Diabetic Medicine : a Journal of the British Diabetic Association·Y LingE J G Sijbrands
Mar 29, 2000·The Journal of Nutrition·P J ReedsF Jahoor
Oct 24, 2006·The Journal of Nutrition·Barry D LambertDouglas G Burrin
Dec 5, 2015·Journal of Cell Communication and Signaling·Soujanya D YelamanchiHarsha Gowda
Aug 4, 1998·The Journal of Nutrition·G Wu
Apr 22, 2010·American Journal of Physiology. Endocrinology and Metabolism·Juan C MariniBrendan Lee
Aug 22, 2008·The Journal of Nutrition·Johannes B van GoudoeverSophie van der Schoor
Jun 27, 2000·The Journal of Nutrition·P J Reeds
Jul 25, 2000·Nutrition·P J Reeds, D G Burrin

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