Proline reverses the abnormal phenotypes of Colletotrichum trifolii associated with expression of endogenous constitutively active Ras.

Applied and Environmental Microbiology
Stephen D MemmottM B Dickman

Abstract

Colletotrichum trifolii is the causative organism of alfalfa anthracnose. We previously cloned and characterized the small prototypical G protein, Ras, of C. trifolii, which is involved in the signaling pathways that mediate interaction between the pathogen and its host. Transformants expressing constitutively active forms of Ras have growth medium-dependent phenotypes. In nutrient-rich media (e.g., yeast extract and peptone), the phenotype of the transformants was indistinguishable from that of the wild type. However, during nutrient starvation, the transformants lose polarity, have distended hyphae, and fail to sporulate and produce appressoria. Since peptone caused the phenotype to revert, amino acids were tested singly and in combination to identify the responsible amino acid(s). We found that 1.6 mM proline in the medium reverses the constitutively active Ras phenotype.

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Citations

Oct 14, 2003·FEMS Microbiology Letters·Young-sil HaMartin B Dickman
Feb 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Changbin Chen, Martin B Dickman
Nov 27, 2007·Free Radical Biology & Medicine·Navasona KrishnanDonald F Becker
May 3, 2016·Seminars in Cell & Developmental Biology·Kou Yanjun, Naweed I Naqvi
Aug 31, 2021·Frontiers in Cell and Developmental Biology·Eduardo J PatriarcaGabriella Minchiotti

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