Proline-rich salivary proteins have extended conformations.

Biophysical Journal
Hélène BozeBernard Cabane

Abstract

Three basic proline-rich salivary proteins have been produced through the recombinant route. IB5 is a small basic proline-rich protein that is involved in the binding of plant tannins in the oral cavity. II-1 is a larger protein with a closely related backbone; it is glycosylated, and it is also able to bind plant tannins. II-1 ng has the same polypeptidic backbone as II-1, but it is not glycosylated. Small angle x-ray scattering experiments on dilute solutions of these proteins confirm that they are intrinsically disordered. IB5 and II-1 ng can be described through a chain model including a persistence length and cross section. The measured radii of gyration (Rg=27.9 and 41.0+/-1 A respectively) and largest distances (rmax=110 and 155+/-10 A respectively) show that their average conformations are rather extended. The length of the statistical segment (twice the persistence length) is b=30 A, which is larger than the usual value (18 A-20 A) for unstructured polypeptide chains. These characteristics are presumably related to the presence of polyproline helices within the polypeptidic backbones. For both proteins, the radius of gyration of the chain cross-section is Rc=2.7+/-0.2A. The glycosylated protein II-1 has similar conform...Continue Reading

References

Jan 1, 1979·Archives of Oral Biology·D L Kauffman, P J Keller
Jul 1, 1992·The International Journal of Biochemistry·T OhoA Hjerpe
Apr 25, 1992·British Dental Journal·W M Edgar
Dec 1, 1985·International Journal of Peptide and Protein Research·R E LoomisL A Tabak
Jan 1, 1968·Biopolymers·P Sharp, V A Bloomfield
Feb 11, 1982·Biochemical and Biophysical Research Communications·M S ReddyL A Tabak
Oct 23, 1998·Archives of Oral Biology·Y Lu, A Bennick
Nov 1, 1993·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·M KallalaB Cabane
Jun 22, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J W DennisC E Warren
Nov 5, 1999·Journal of Molecular Biology·P E Wright, H J Dyson
May 31, 2001·Journal of Molecular Graphics & Modelling·A K DunkerZ Obradovic
Mar 23, 2002·Protein Science : a Publication of the Protein Society·Vladimir N Uversky
Mar 13, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Cécile SimonErick J Dufourc
Aug 26, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Peter Tompa
May 11, 2004·Biomacromolecules·Elisabeth JöbstlMike P Williamson
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·H Jane Dyson, Peter E Wright
Jun 1, 2005·Biophysical Journal·Maxim V Petoukhov, Dmitri I Svergun
Oct 6, 2005·Biochimica Et Biophysica Acta·Wei Gu, Volkhard Helms
Oct 26, 2005·Biophysical Chemistry·Mikulás Bánó, Jozef Marek
Nov 16, 2005·Proteins·Véronique Receveur-BréchotSonia Longhi
Mar 15, 2006·Protein Expression and Purification·Christine PascalPascale Sarni-Manchado
Jul 18, 2006·Nucleic Acids Research·Julien MaupetitPierre Tufféry
Apr 7, 2007·Journal of the American Chemical Society·Pau BernadóDmitri I Svergun
Jul 27, 2007·Journal of Dental Research·E J Helmerhorst, F G Oppenheim
Jul 23, 2008·Journal of Agricultural and Food Chemistry·Christine PascalAude Vernhet
Sep 24, 2008·Journal of Agricultural and Food Chemistry·Pascale Sarni-ManchadoVéronique Cheynier
Jul 15, 2009·Cell Research·Bo HeA Keith Dunker
Oct 20, 2009·Analytical and Bioanalytical Chemistry·Francis CanonPascale Sarni-Manchado
Nov 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jintao LiuCarlos J Camacho

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Citations

Jul 29, 2010·Analytical and Bioanalytical Chemistry·Francis CanonPascale Sarni-Manchado
Apr 10, 2013·Journal of Agricultural and Food Chemistry·Jennifer A Barak, James A Kennedy
Dec 23, 2011·International Journal of Dentistry·Martin Levine
Mar 8, 2013·Annual Review of Food Science and Technology·Guy H Carpenter
Jun 27, 2012·Progress in Retinal and Eye Research·Lei Zhou, Roger W Beuerman
May 2, 2014·Proteomics·Natarajan PerumalFranz H Grus
Apr 17, 2015·Journal of Agricultural and Food Chemistry·Jacqui M McRaePaul A Smith
Jun 13, 2014·Colloids and Surfaces. B, Biointerfaces·Hannah L GibbinsGuy H Carpenter
Sep 8, 2012·Current Opinion in Structural Biology·Javier Pérez, Yoshinori Nishino
Oct 30, 2016·Current Opinion in Structural Biology·Tiago N CordeiroPau Bernadó
Jan 19, 2018·Annual Review of Biophysics·Alex S Holehouse, Rohit V Pappu
Apr 17, 2012·Chembiochem : a European Journal of Chemical Biology·Nasrollah Rezaei-GhalehMarkus Zweckstetter
Mar 15, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jacqui M McRae, James A Kennedy
May 16, 2011·Molecular Informatics·Jérôme GolebiowskiSerge Antonczak
Oct 9, 2013·Acta Crystallographica. Section D, Biological Crystallography·Bodo SanderHermann Schindelin
Jun 20, 2018·International Journal of Molecular Sciences·Gea GuerrieroKhawar Sohail Siddiqui
Sep 29, 2011·Molecular BioSystems·Pau Bernadó, Dmitri I Svergun
Jun 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Ivan PeranDaniel P Raleigh
Apr 18, 2019·Proceedings of the National Academy of Sciences of the United States of America·Joshua A RibackTobin R Sosnick
Jan 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joseph CarmichealSurinder K Batra
Aug 5, 2017·Nutrition & Metabolism·Nicole M DelimontBrian L Lindshield
Mar 16, 2018·Oncology Letters·Seda EkizogluNur Buyru
Aug 5, 2017·Biotechnology and Applied Biochemistry·Darragh P O'BrienPatrice Vachette
Oct 21, 2020·Emerging Topics in Life Sciences·Erik W Martin, Alex S Holehouse
Jan 19, 2020·Biophysical Journal·Giulia SalzanoGabriele Giachin
Feb 12, 2020·Journal of Molecular Biology·Patricia L ClarkTobin R Sosnick
Nov 11, 2020·Journal of Agricultural and Food Chemistry·Carlos GuerreiroSusana Soares
Nov 18, 2015·Journal of Chemical Theory and Computation·João HenriquesMarie Skepö
Jan 10, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Francis CanonBernard Cabane
Apr 29, 2011·Journal of the American Chemical Society·Francis CanonPhilippe Dugourd

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