Prolongation of carrageenan-induced inflammation in human colonic epithelial cells by activation of an NFκB-BCL10 loop.

Biochimica Et Biophysica Acta
Alip BorthakurJoanne K Tobacman

Abstract

Carrageenan, a sulfated polysaccharide that is widely used as a food additive, induces inflammatory responses in animal models and human cells. The carrageenan-induced inflammatory cascades involve toll-like receptor (TLR)4- and B-cell leukemia/lymphoma (BCL)10-dependent activation of NF-κB, leading to increased IL-8 production. Translocations involving BCL10 in the mucosa-associated lymphoid tissue (MALT) lymphomas are associated with constitutive activation of NF-κB. This report presents a mechanism by which carrageenan exposure leads to prolonged activation of both BCL10 and NF-κB in human colonic epithelial cells. Study findings demonstrate that nuclear RelA and RelB bind to an NF-κB binding motif in the BCL10 promoter in human colonic epithelial NCM460 and HT-29 cells. In vitro oligonucleotide binding assay, non-radioactive gel shift assay, and chromatin immunoprecipitation (ChIP) indicate binding of RelA and RelB to the BCL10 promoter. Prolonged inflammation follows activation of the BCL10-NFκB inflammatory loop in response to carrageenan, shown by increased BCL10, RelA, and IL-8 for 36 to 48h and increased RelB for 24h following withdrawal of carrageenan after 12h. In contrast, exposure to dextran sulfate sodium, which d...Continue Reading

Citations

May 17, 2013·Journal of Physiology and Biochemistry·Wu XuStephan W Morris
Oct 29, 2013·Human Molecular Genetics·Nora Fernandez-JimenezJose Ramon Bilbao
Jun 15, 2013·Mediators of Inflammation·Sumit BhattacharyyaJoanne K Tobacman
Jun 8, 2014·Inflammopharmacology·Nidhi GoyalPuneet Kumar
Dec 1, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Myra L Weiner
Aug 11, 2015·Carbohydrate Polymers·Sónia S FerreiraManuel A Coimbra
Feb 3, 2015·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·James M McKimWilliam R Blakemore
Aug 18, 2016·Frontiers in Immunology·Abigail BassonFabio Cominelli
Jun 28, 2017·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Myra L WeinerWilliam R Blakemore
Mar 30, 2018·Applied Microbiology and Biotechnology·Muhammad OvaisZabta Khan Shinwari
Feb 9, 2020·Journal of Clinical Pathology·Norris Hollie, Saja Asakrah
Aug 23, 2017·Drug Delivery and Translational Research·Joshi Poorvashree, Dhaneshwar Suneela
Jun 20, 2021·Comprehensive Reviews in Food Science and Food Safety·Fang LiuRobert W Li
Jul 21, 2021·Bioengineered·Yu-Chen LiaoJo-Shu Chang

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