PMID: 22586916May 17, 2012Paper

Prolonged cultivation of an anaerobic bacterial community producing hydrogen

Prikladnaia biokhimiia i mikrobiologiia
B F BelokopytovV A Shcherbakova

Abstract

This paper studies various methods of long-term maintenance of the process of hydrogen evolution during the growth of an aerobic bacterial community on a starch-containing environment. When cultured in separable trip fermentation mode for 72 days, from 0.10 to 0.23 H2/l of medium/day was formed. The regime of regular reseeding lasted more than 100 days, forming an average of 0.81 1 H2/l of medium/day. The advantages and disadvantages of different methods of microbial hydrogen production during a dark starch fermentation process are presented. From the obtained H2 forming microbial communities, we isolated an anaerobic spore-forming bacterium (strain BF). Phylogenetic analysis of the 16S RNA gene sequence of the new strain showed that according to its genotype it belongs to the Clostridium butyricum species.

References

May 10, 2002·Biotechnology and Bioengineering·Sergey KosourovMaria L Ghirardi
Oct 11, 2003·Journal of Environmental Management·Tong ZhangHerbert H P Fang
Oct 20, 2005·Journal of Bioscience and Bioengineering·Guangzhen Liu, Jianquan Shen
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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