Prolonged Dye Release from Mesoporous Silica-Based Imaging Probes Facilitates Long-Term Optical Tracking of Cell Populations In Vivo

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Jessica M RosenholmCecilia Sahlgren

Abstract

Nanomedicine is gaining ground worldwide in therapy and diagnostics. Novel nanoscopic imaging probes serve as imaging tools for studying dynamic biological processes in vitro and in vivo. To allow detectability in the physiological environment, the nanostructure-based probes need to be either inherently detectable by biomedical imaging techniques, or serve as carriers for existing imaging agents. In this study, the potential of mesoporous silica nanoparticles carrying commercially available fluorochromes as self-regenerating cell labels for long-term cellular tracking is investigated. The particle surface is organically modified for enhanced cellular uptake, the fluorescence intensity of labeled cells is followed over time both in vitro and in vivo. The particles are not exocytosed and particles which escaped cells due to cell injury or death are degraded and no labeling of nontargeted cell populations are observed. The labeling efficiency is significantly improved as compared to that of quantum dots of similar emission wavelength. Labeled human breast cancer cells are xenotransplanted in nude mice, and the fluorescent cells can be detected in vivo for a period of 1 month. Moreover, ex vivo analysis reveals fluorescently labele...Continue Reading

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Citations

Sep 12, 2017·Advanced Healthcare Materials·Cecilia SahlgrenNiklas Sandler
Feb 2, 2018·Advanced Materials·Didem Şen KaramanAnu J Airaksinen
Nov 28, 2019·Scientific Reports·Nithiya Nirmalananthan-BudauUte Resch-Genger
Dec 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lei WangRuiyuan Liu
Feb 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Wanfu XuRuiyuan Liu
Dec 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jixi Zhang, Kaiyong Cai
Aug 11, 2021·Pharmaceutics·Elham RastegariYueh Chien
Feb 6, 2017·Bioconjugate Chemistry·Jun LiH Charles Manning
Dec 17, 2016·Chemical Research in Toxicology·Bella B ManshianStefaan J Soenen

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