Prolonged exposure of human neuroblastoma SH-SY5Y cell line to morphine and oxotremorine modulates signal transduction in adenylate cyclase system

Brain Research
M Olasmaa, L Terenius

Abstract

Neurotransmitter receptors coupling to the adenylate cyclase (AC) system were studied in the human neuroblastoma SH-SY5Y cell line. Vasoactive intestinal polypeptide (VIP) caused an up to 40-fold enhancement of the AC activity, while prostaglandin E1 (PGE1) was able to increase the cAMP accumulation 2.5-fold. Stimulation either by VIP or PGE1 was attenuated with either morphine (MOR) or oxotremorine (OXO). Prolonged exposure to MOR and OXO caused a ligand-specific, i.e. homologous desensitization of the opioid and muscarinic acetylcholine receptors, respectively. Preceding desensitization, a supersensitive response of the AC system to VIP was observed. Pretreatment of cells with PT overnight reduced the inhibitory effects of both MOR and OXO. Nevertheless, in cells pretreated with PT and then also with OXO, MOR and OXO inhibited the VIP-induced AC response. Apparently, there are both PT-sensitive and -insensitive pathways to AC inhibition in SH-SY5Y cells.

References

Jun 15, 1975·Life Sciences·W A KleeM Nirenberg
Jun 13, 1986·Biochemical and Biophysical Research Communications·S M Kazmi, R K Mishra
Jan 21, 1985·Neuroscience Letters·M Olasmaa, L Terenius
Apr 1, 1974·Analytical Biochemistry·Y SalomonM Rodbell
Oct 1, 1963·The Biochemical Journal·F C GREENWOODJ S GLOVER

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.