Prolonged pre-incubation increases the susceptibility of Galleria mellonella larvae to bacterial and fungal infection

Virulence
Niall BrowneKevin Kavanagh

Abstract

Galleria mellonella larvae are widely used for assessing the virulence of microbial pathogens and for measuring the in vivo activity of antimicrobial agents and produce results comparable to those that can be obtained using mammals. The aim of the work described here was to ascertain the effect of pre-incubation at 15°C for 1, 3, 6 or 10 weeks on the susceptibility of larvae to infection with Candida albicans and Staphylococcus aureus. Larvae infected with C. albicans after 1 week pre-incubation at 15°C showed 73.3 ± 3.3% survival at 24 hours post-infection while those infected after 10 weeks pre-incubation showed 30 ± 3.3% survival (P < 0.01). Larvae infected with S. aureus after 1 week pre-incubation showed 65.5 ± 3.3% survival after 24 hours while those infected after 10 weeks pre-incubation showed 13.3 ± 3.3% (P < 0.001). Analysis of the haemocyte density in larvae pre-incubated for 3-10 weeks showed a reduction in haemocytes over time but a proportionate increase in the density of granular haemocytes in the population as determined by FACS analysis. Proteomic analysis revealed decreased abundance of proteins associated with metabolic pathways (e.g. malate dehydrogenase, fructose-1,6-bisphosphatase, glyceraldehyde-3-phospha...Continue Reading

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Citations

Apr 24, 2015·Virulence·Andrew P Desbois, Stuart McMillan
Apr 3, 2019·Journal of Fungi·Ilse D Jacobsen
Sep 25, 2020·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Monalessa Fábia Pereira, Ciro César Rossi
Sep 23, 2020·Pathogens and Disease·Monalessa Fábia PereiraDenise Mara Soares Bazzolli
Feb 26, 2021·F1000Research·Nicola J Senior, Richard W Titball
Aug 20, 2021·Frontiers in Cellular and Infection Microbiology·Patrícia Pimentel de BarrosJuliana Campos Junqueira

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Methods Mentioned

BETA
FACS
Fluorescence-activated cell sorting
electrophoresis

Software Mentioned

Progenesis SameSpot
Blast2GO
MaxQuant
GraphPad
MASCOT
Andromeda
Progenesis
Persues
GraphPad Prism

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