Prolonged reduction of leukocyte membrane-associated Dectin-1 levels following beta-glucan administration

The Journal of Pharmacology and Experimental Therapeutics
Tammy Ozment-SkeltonDavid L Williams

Abstract

Dectin-1 is the primary pattern recognition receptor for fungal glucans. Dectin-1 mediates the internalization and biological response to glucans. We examined the effect of i.v. or i.p. glucan phosphate (GP) administration on Dectin-1 membrane expression in murine peripheral blood leukocytes, splenocytes, bone marrow, and peritoneal cells from 3 h to 10 days after injection. Circulating leukocytes were also examined for uptake and internalization of glucans from the blood. Fluorescent-labeled GP was taken up from the systemic circulation by circulating peripheral leukocytes, splenocytes, and peritoneal cells. Following internalization, glucan colocalized with Dectin-1 in an intracellular vesicle. A single parenteral injection of GP resulted in a significant reduction (approximately 33-85%) in peripheral leukocyte membrane-associated Dectin-1 positivity that lasted for up to 7 days. The loss of leukocyte membrane-associated Dectin-1 after GP administration was primarily due to decreased levels of Dectin-1 on neutrophil and monocyte membranes with no significant changes in the percentage of neutrophils or monocytes circulating in the blood. Administration of control carbohydrate polymers, i.e., mannan or pullulan, which are not l...Continue Reading

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Citations

Jul 9, 2008·Medical Mycology·Matti Kimberg, Gordon D Brown
Feb 25, 2009·Critical Care Medicine·Tammy R Ozment-SkeltonDavid L Williams
Jun 16, 2012·The Journal of Pharmacology and Experimental Therapeutics·Tammy R OzmentDavid L Williams
Aug 25, 2007·American Journal of Respiratory Cell and Molecular Biology·Margaret M KellyPaul Kubes
Jun 7, 2013·International Journal of Oncology·Luca VannucciMustafa Saieh
Apr 28, 2009·Neuroscience Letters·Vaibhav B ShahLakhu Keshvara
Jul 29, 2011·The Journal of Investigative Dermatology·Carren Sy HauShinichi Sato
Dec 17, 2008·Annals of the New York Academy of Sciences·S Vicky Tsoni, Gordon D Brown
Jan 9, 2009·European Journal of Immunology·Patricia Hernanz-FalcónCaetano Reis e Sousa
Feb 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vaibhav B ShahLakhu Keshvara

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