Prolonged survival of Serratia marcescens in chlorhexidine.

Applied and Environmental Microbiology
T J Marrie, J W Costerton

Abstract

During an outbreak of Serratia marcescens infections at our hospital, we discovered widespread contamination of the 2% chlorhexidine hand-washing solution by S. marcescens. Examination by electron microscopy of the sides of bottles in which this solution was stored revealed that microorganisms were embedded in a fibrous matrix. Bacteria, free in the liquid, were morphologically abnormal, showing cell wall disruption or cytoplasmic changes. Furthermore, bacteria adherent to the walls of the storage jugs and embedded in this fibrous matrix also had morphologically abnormal cytoplasm. Despite these changes, viable S. marcescens organisms were recovered from the fluid during a storage period of 27 months. The concentration of chlorhexidine required to inhibit these strains of Serratia was 1,024 microgram/ml; however, the organism could survive in concentrations of up to 20,000 micrograms/ml. Additional studies are needed to define the mechanism(s) that allows such bacteria to contaminate and survive in disinfectants.

References

Dec 1, 1977·Journal of Clinical Periodontology·T D Hennessey
Feb 1, 1977·Journal of Clinical Microbiology·D J Lovell, D J Bibel
Apr 15, 1967·British Medical Journal·D W Burdon, J L Whitby

❮ Previous
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Citations

Mar 1, 1990·The British Journal of Ophthalmology·D F LarkinD L Easty
Jan 1, 1990·American Journal of Public Health·R L AndersonM S Favero
Aug 1, 1996·The British Journal of Ophthalmology·J Hay, D V Seal
Jul 8, 1999·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·H Peltroche-LlacsahuangaG Haase
May 13, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Albert T Sheldon
Feb 21, 2009·Revista latino-americana de enfermagem·Christiane Moreira PadovaniVânia Regina Goveia
Oct 7, 2011·Clinical Microbiology Reviews·Steven D Mahlen
Aug 16, 2019·Antimicrobial Resistance and Infection Control·Laura MolesJuan M Rodríguez
Jan 21, 2020·Interactive Cardiovascular and Thoracic Surgery·Angel L FernándezJulian Alvarez Escudero
Apr 1, 1985·European Journal of Clinical Microbiology·J C NickelJ W Costerton
Jul 1, 1990·Archiv für Tierernährung·M VanbelleM Focant
Oct 19, 2004·Clinical Microbiology Reviews·Günter Kampf, Axel Kramer
Feb 1, 1990·The Journal of Dairy Research·T MattilaA L Kyläsiurola
Nov 1, 1989·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D SticklerJ Chawla
Oct 3, 2007·Antimicrobial Agents and Chemotherapy·David J WeberEmily E Sickbert-Bennett
Dec 6, 2000·Revista de saúde pública·F MiyagiF Alterthum
Apr 15, 2003·Clinical Microbiology Reviews·Peter Gilbert, Andrew J McBain
Jan 8, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·John M BoyceUNKNOWN Healthcare Infection Control Practices Advisory Committee. Society for Healthcare Epidemiology of America. Association for P
Dec 12, 2012·Expert Opinion on Investigational Drugs·Divya Prakash GnanadhasDipshikha Chakravortty
Apr 17, 2007·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Emma B H HumeMark D P Willcox
Nov 16, 2019·Acta Veterinaria Scandinavica·Mari Johanna FrimanMerja Hilma Johanna Rantala
Mar 26, 2021·Microbial Pathogenesis·Martín A González-MontalvoAngel Andrade
Nov 21, 2000·Colloids and Surfaces. B, Biointerfaces·G WirtanenT Mattila-Sandholm
May 19, 2001·The Journal of Hospital Infection·S M MurtoughA D Russell
Aug 1, 1984·Applied and Environmental Microbiology·M E McBride
Jan 9, 1999·Clinical Microbiology Reviews·G McDonnell, A D Russell
Oct 1, 1984·Journal of Clinical Microbiology·T OkudaH Zen-Yoji
Jun 1, 1988·Antimicrobial Agents and Chemotherapy·J FreneyJ Fleurette
Jan 1, 1984·Infection and Immunity·J MillsJ W Costerton
Feb 1, 1985·Applied and Environmental Microbiology·C M Llabres, D G Ahearn
Jul 1, 1996·The Journal of Hospital Infection·C BosiC Bollet

❮ Previous
Next ❯

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