Prolonged weightlessness affects promyelocytic multidrug resistance

Experimental Cell Research
E H PiepmeierM L Lewis

Abstract

An immortalized promyelocytic cell line was studied to detect how doxorubicin uptake is affected by microgravity. The purpose of this experiment was to identify the effect that microgravity may have on multidrug resistance in leukocytes. HL60 cells and HL60 cells resistant to anthracycline (HL60/AR) were grown in RPMI and 10% FBS. Upon reaching orbit in the Space Shuttle Endeavour, the cells were robotically mixed with doxorubicin. Three days after mixing, cells were fixed with paraformaldehyde/glutaraldehyde. Ground control experiments were conducted concurrently using a robot identical to the one used on the Shuttle. Fixed cells were analyzed within 2 weeks of launch. Confocal micrographs identified changes in cell structure (transmittance), drug distribution (fluorescence), and microtubule polymerization (fluorescence). Flight cells showed a lack of cytoskeletal polymerization resulting in an overall amorphic globular shape. Doxorubicin distribution in ground cells included a large numbers of vesicles relative to flight cells. There was a greater amount of doxorubicin present in flight cells (85% +/- 9.7) than in ground control cells (43% +/- 26) as determined by image analysis. Differences in microtubule formation between f...Continue Reading

References

Nov 1, 1991·Experimental Cell Research·M LimouseL Schaffar
Jul 1, 1987·The Journal of Cell Biology·D R WebsterG G Borisy
Jul 13, 1984·Science·A CogoliP Fuchs-Bislin
Apr 10, 1996·Experimental Cell Research·M Hughes-Fulford, M L Lewis

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Citations

Apr 16, 2013·Nature Reviews. Cancer·Jeanne L Becker, Glauco R Souza
Aug 26, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Claudia UlbrichDaniela Grimm
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M L LewisP D Bowman
Jul 3, 2019·International Journal of Molecular Sciences·Mohamed Zakaria NassefDaniela Grimm
Aug 26, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M L LewisE H Piepmeier

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