Prolyl Endopeptidase (PREP) is Associated With Male Reproductive Functions and Gamete Physiology in Mice

Journal of Cellular Physiology
Raffaele DotoloSabrina Diano

Abstract

Prolyl endopeptidase (PREP) is a serine protease which has been implicated in many biological processes, such as the maturation and degradation of peptide hormones and neuropeptides, learning and memory, cell proliferation and differentiation, and glucose metabolism. A small number of reports have also suggested PREP participation in both male and female reproduction-associated processes. In the present work, we examined PREP distribution in male germ cells and studied the effects of its knockdown (Prep(gt/gt) ) on testis and sperm in adult mice. The protein is expressed and localized in elongating spermatids and luminal spermatozoa of wild type (wt) mice, as well as Sertoli, Leydig, and peritubular cells. PREP is also expressed in the head and midpiece of epididymal spermatozoa, whereas the remaining tail region shows a weaker signal. Furthermore, testis weight, histology of seminiferous tubules, and epididymal sperm parameters were assessed in wt and Prep(gt/gt) mice: wild type testes have larger average tubule and lumen diameter; in addition, lumenal composition of seminiferous tubules is dissimilar between wt and Prep(gt/gt) , as the percentage of spermiated tubules is much higher in wt. Finally, total sperm count, sperm mo...Continue Reading

References

Nov 28, 1983·Life Sciences·S Wilk
Jun 1, 1980·Analytical Biochemistry·W L TaylorJ E Dixon
May 20, 1998·Molecular Reproduction and Development·A KimuraT Takahashi
Jan 17, 2002·Neurochemistry International·Jon IrazustaLuis Casis
Jun 23, 2004·European Journal of Biochemistry·Jarkko I VenäläinenPekka T Männistö
Sep 25, 2004·Journal of Cellular Physiology·Scott B HerrickRichard A Cardullo
Sep 28, 2005·Neurochemical Research·Steffen RossnerHans-Ulrich Demuth
May 17, 2006·Advances in Experimental Medicine and Biology·Joachim Wolfgang BärHans-Ulrich Demuth
Jun 24, 2006·Chemistry & Biology·Matthew SiegelChaitan Khosla
Mar 14, 2008·Current Protein & Peptide Science·Zoltán Szeltner, László Polgár
Jun 29, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Timo T MyöhänenOlli Carpen
Jan 26, 2013·The International Journal of Biochemistry & Cell Biology·Takashi MatsudaMasanori Takaoka
Oct 25, 2013·Nutrients·Isabel CominoCarolina Sousa
Nov 26, 2013·Biochemical and Biophysical Research Communications·Kanayo SuzukiMasanori Takaoka
Jul 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jung Dae KimSabrina Diano

❮ Previous
Next ❯

Citations

Nov 1, 2018·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Massimo Venditti, Sergio Minucci
Apr 17, 2019·Molecular Reproduction and Development·Ping XuChunping Zhang
May 8, 2019·Zygote : the Biology of Gametes and Early Embryos·Massimo VendittiSergio Minucci
Dec 23, 2015·BMC Genomics·Tao HuangYu-Dong Cai
Feb 11, 2020·Systems Biology in Reproductive Medicine·Wenyang ChenGuirong Zhang
Apr 25, 2020·Molecular Reproduction and Development·Massimo VendittiImed Messaoudi
Aug 8, 2021·International Journal of Molecular Sciences·Massimo VendittiSergio Minucci

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.