Promethazine improves antibiotic efficacy and disrupts biofilms of Burkholderia pseudomallei

Biofouling
José Júlio Costa SidrimRaimunda Sâmia Nogueira Brilhante

Abstract

Efflux pumps are important defense mechanisms against antimicrobial drugs and maintenance of Burkholderia pseudomallei biofilms. This study evaluated the effect of the efflux pump inhibitor promethazine on the structure and antimicrobial susceptibility of B. pseudomallei biofilms. Susceptibility of planktonic cells and biofilms to promethazine alone and combined with antimicrobials was assessed by the broth microdilution test and biofilm metabolic activity was determined with resazurin. The effect of promethazine on 48 h-grown biofilms was also evaluated through confocal and electronic microscopy. The minimum inhibitory concentration (MIC) of promethazine was 780 mg l-1, while the minimum biofilm elimination concentration (MBEC) was 780-3,120 mg l-1. Promethazine reduced the MIC values for erythromycin, trimethoprim/sulfamethoxazole, gentamicin and ciprofloxacin and reduced the MBEC values for all tested drugs (p<0.05). Microscopic analyses demonstrated that promethazine altered the biofilm structure of B. pseudomallei, even at subinhibitory concentrations, possibly facilitating antibiotic penetration. Promethazine improves antibiotics efficacy against B. pseudomallei biofilms, by disrupting biofilm structure.

References

Feb 27, 1999·Antimicrobial Agents and Chemotherapy·R A MooreD E Woods
Apr 3, 2001·International Journal of Antimicrobial Agents·M Viveiros, L Amaral
Mar 30, 2004·Antimicrobial Agents and Chemotherapy·Y Y ChanK L Chua
Oct 29, 2004·The Journal of Antimicrobial Chemotherapy·F M ThibaultJ-D Cavallo
Dec 6, 2006·Antimicrobial Agents and Chemotherapy·Ying Ying ChanKim Lee Chua
Oct 7, 2008·Applied and Environmental Microbiology·Malin KvistPer Klemm
Feb 13, 2010·International Journal of Antimicrobial Agents·Niels HøibyOana Ciofu
Oct 23, 2010·The Journal of Antimicrobial Chemotherapy·Takehiko MimaZe-Qi Xu
Feb 1, 2012·Antimicrobial Agents and Chemotherapy·R S N BrilhanteJ J C Sidrim
Feb 23, 2012·Recent Patents on Anti-infective Drug Discovery·Ashima K Bhardwaj, Priyabrata Mohanty
Sep 14, 2012·The New England Journal of Medicine·W Joost WiersingaSharon J Peacock
Nov 2, 2013·The Journal of Antimicrobial Chemotherapy·Stephanie BaughMark A Webber
Mar 8, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D LimmathurotsakulS J Peacock
Mar 13, 2014·International Journal of Antimicrobial Agents·David Dance
Mar 25, 2014·Current Opinion in Microbiology·Cristina SolanoIñigo Lasa
Feb 1, 2015·International Journal of Antimicrobial Agents·Seiji YamasakiKunihiko Nishino

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Citations

Aug 31, 2019·Future Microbiology·José Jc SidrimDébora de Scm Castelo-Branco
Dec 23, 2020·Biofouling·Débora de Souza Collares Maia Castelo-BrancoJosé Júlio Costa Sidrim
Feb 9, 2021·Journal of Applied Microbiology·Gláucia Morgana de Melo GuedesDébora de Souza Collares Maia Castelo-Branco

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Methods Mentioned

BETA
electron microscopy
scanning electron microscopy
phase contrast
confocal microscopy

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