Prominent expression of phosphodiesterase 5 in striated muscle of the rat urethra and levator ani.

The Journal of Urology
Guiting LinChing-Shwun Lin

Abstract

We investigated phosphodiesterase 5 distribution and activity in the urethra. Rat tissues were examined for phosphodiesterase 5 and alpha-smooth muscle actin expression. Urethral phosphodiesterase 5 activity was examined by tissue bath in the presence of sildenafil (Pfizer, New York, New York). Anti-alpha-smooth muscle actin antibody (Abcam) stained all known smooth muscles in all tested tissues and revealed a few smooth muscle fibers in the levator ani muscle. Anti-phosphodiesterase 5 antibody (Abcam) stained smooth muscle in the penis and bladder but not striated leg muscle. However, it stained predominantly striated muscle in the urethra and the levator ani muscle. In the urethra the amount of phosphodiesterase 5 in striated muscle was 6 times that in smooth muscle. In urethral striated muscle phosphodiesterase 5 expression was localized to Z-band striations. Smooth and striated muscle intermingling was clearly visible on the inner and outer rims of the circularly arranged striated muscle layer. Relaxation of precontracted urethral tissues by sodium nitroprusside (Sigma-Aldrich) was enhanced by sildenafil, indicating phosphodiesterase 5 activity, which was primarily located in the striated muscle according to phosphodiestera...Continue Reading

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Citations

Jul 6, 2014·International Urogynecology Journal·Hideaki IzumiYoichi Arai
Mar 1, 2012·Lower Urinary Tract Symptoms·Chen Zhao, Jong Kwan Park
Apr 23, 2016·The American Journal of Pathology·Jennifer TinklenbergMichael W Lawlor
Mar 4, 2015·Urology·Lin WangChing-Shwun Lin
Aug 31, 2019·Current Urology Reports·Hanson Zhao, Howard H Kim
Dec 17, 2016·The Journal of Urology·Allen D Seftel

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