Promiscuous target interactions in the mariner transposon Himar1.

The Journal of Biological Chemistry
Karen LipkowRonald Chalmers

Abstract

We have previously characterized the early intermediates of mariner transposition. Here we characterize the target interactions that occur later in the reaction. We find that, in contrast to the early transposition intermediates, the strand transfer complex is extremely stable and difficult to disassemble. Transposase is tightly bound to the transposon ends constraining rotation of the DNA at the single strand gaps in the target site flanking the element on either side. We also find that although the cleavage step requires Mg2+ or Mn2+ as cofactor, the strand transfer step is also supported by Ca2+, suggesting that the structure of the active site changes between cleavage and insertion. Finally, we show that, in contrast to the bacterial cut and paste transposons, mariner target interactions are promiscuous and can take place either before or after cleavage of the flanking DNA. This is similar to the behavior of the V(D)J system, which is believed to be derived from an ancestral eukaryotic transposon. We discuss the implications of promiscuous target interactions for promoting local transposition and whether this is an adaptation to facilitate the invasion of a genome following horizontal transfer to a new host species.

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Citations

Dec 30, 2008·Molecular Genetics and Genomics : MGG·Gwénaëlle CrénèsAgnès Petit
Aug 4, 2009·Genetica·Corentin Claeys Bouuaert, Ronald M Chalmers
Dec 31, 2008·Journal of Bacteriology·Paul A BeareRobert A Heinzen
Nov 3, 2010·Molecular and Cellular Biology·Corentin Claeys BouuaertRonald Chalmers
Nov 30, 2006·Molecular and Cellular Biology·Danxu LiuRonald Chalmers
Jul 25, 2009·Progress in Biophysics and Molecular Biology·Mark A DePristoKaren Lipkow
Aug 5, 2008·Journal of Molecular Biology·Martín Muñoz-LópezTeresa Palomeque
Jun 26, 2013·ELife·Corentin Claeys BouuaertRonald Chalmers
Nov 26, 2013·The Journal of Biological Chemistry·Aude PfliegerSylvaine Renault
Aug 21, 2019·Nucleic Acids Research·Shivam Bhatt, Ronald Chalmers
Apr 3, 2010·Biochemistry·Sylvaine RenaultCorinne Augé-Gouillou

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