Promising insights into the kosmotropic effect of magnetic nanoparticles on proteins: The pivotal role of protein corona formation.

Biotechnology Progress
Reza FattahAhmad Fazeli

Abstract

Increasing concerns about biosafety of nanoparticles (NPs) has raised the need for detailed knowledge of NP interactions with biological molecules especially proteins. Herein, the concentration-dependent effect of magnetic NPs (MNPs) on bovine serum albumin and hen egg white lysozyme was explored. The X-ray diffraction patterns, zeta potential, and dynamic light scattering measurements together with scanning electron microscopy images were employed to characterize MNPs synthesized through coprecipitation method. Then, we studied the behavior of two model proteins with different surface charges and structural properties on interaction with Fe3 O4 . A thorough investigation of protein-MNP interaction by the help of intrinsic fluorescence at different experimental conditions revealed that affinity of proteins for MNPs is strongly affected by the similarity of protein and MNP surface charges. MNPs exerted structure-making kosmotropic effect on both proteins under a concentration threshold; however, binding strength was found to determine the extent of stabilizing effect as well as magnitude of the concentration threshold. Circular dichroism spectra showed that proteins with less resistance to conformational deformations are more pr...Continue Reading

References

Jul 16, 1992·Nature·X M He, D C Carter
Nov 16, 1987·FEBS Letters·G V SemisotnovO B Ptitsyn
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·T ImotoF Tanaka
Dec 16, 1998·Journal of Colloid and Interface Science·T J SuJ Penfold
Aug 28, 2003·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Massimo Stefani, Christopher M Dobson
Jan 18, 2005·International Journal of Pharmaceutics·Wei Wang
Sep 21, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Scott H BrewerStefan Franzen
May 31, 2006·Chemical Communications : Chem Comm·Joseph W E WorrallVincent M Rotello
Feb 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tommy CedervallSara Linse
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Vicki L Colvin, Kristen M Kulinowski
Nov 21, 2007·Colloids and Surfaces. B, Biointerfaces·Willem Norde
Mar 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Hans FrauenfelderRobert D Young
Jun 16, 2009·Journal of Molecular Biology·Kell K AndersenDaniel Otzen
Jun 16, 2009·Nature Materials·Andre E NelMike Thompson
Aug 15, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Wai Keen ChungSteven M Cramer
Dec 17, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Soumyananda ChakrabortiPinak Chakrabarti
Mar 9, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Francesco TurciBice Fubini
Jun 18, 2010·ACS Nano·Eudald CasalsVictor Puntes
Sep 15, 2010·Molecular Pharmaceutics·Weiwei GaoOmid C Farokhzad
Nov 3, 2010·Current Opinion in Chemical Biology·Subinoy RanaVincent M Rotello
Nov 17, 2010·Biochimie·Debasish Sen, Dipak K Mandal
Jun 29, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Sugam KumarJoachim Kohlbrecher
Jul 7, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Bhuvnesh BhartiGerhard H Findenegg
Aug 9, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Shihui Li, Daojin Li
Nov 25, 2011·ACS Applied Materials & Interfaces·Anindita MukhopadhyayGoutam De
May 9, 2012·Journal of Colloid and Interface Science·Harasit Kumar MandalAmbikesh Mahapatra
May 9, 2012·International Journal of Biological Macromolecules·Peng QuMaotian Xu
Jul 23, 2013·Journal of Nanobiotechnology·Shruti R SaptarshiAndreas L Lopata
Sep 21, 2013·The Journal of Physical Chemistry. B·Soumyananda ChakrabortiPinak Chakrabarti
Jan 31, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Sugam KumarP Callow
Apr 16, 2014·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Indresh YadavJ Kohlbrecher
Aug 1, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Sumit MehanJoachim Kohlbrecher
Dec 3, 2014·Physical Chemistry Chemical Physics : PCCP·Bo WangPu Chun Ke
Dec 10, 2014·International Journal of Molecular Sciences·Amar B T Ghisaidoobe, Sang J Chung
Jun 5, 2015·Physical Chemistry Chemical Physics : PCCP·Chandrima JashGopinatha Suresh Kumar
Jul 26, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Olga KoshkinaAnnabelle Bertin

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