Promoter analysis of interleukin 19

Biochemical and Biophysical Research Communications
Po-Jen ChenMing-Shi Chang

Abstract

Interleukin (IL)-19 belongs to the IL-10 family which includes IL-19, IL-20, IL-22, AK155, and MDA-7. IL-10 is a potent immunomodulatory cytokine with implications for pathogenesis in various autoimmune diseases. Polymorphism of the IL-10 promoter region correlates with disease outcome. To understand the gene regulation of IL-19, we analyzed the IL-19 promoter region. A regulatory region (PE), 148bp upstream of exon 1 of IL-19 and linked to a luciferase reporter gene, supported luciferase activity 13 times greater than that supported by a negative promoterless control. An electrophoretic mobility shift assay (EMSA) showed specific binding sites for the transcription factors of the oligonucleotides PE1 (-148 to -98) derived from PE. We identified the sequence TGTGGT (-142 to -138) on PE1 as the binding site for the transcription factor AML1, and crucial for the promoter activity of IL-19 because substituting 1bp in the PE region (-139G-->T) abolished IL-19 promoter activity.

References

May 4, 2004·International Immunopharmacology·Grant GallagherRaymond P Donnelly
Apr 14, 2005·European Journal of Immunology·William J JordanGrant Gallagher

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Citations

Sep 25, 2012·Human Immunology·Dor Mohammad Kordi-TamandaniAdam Torkamanzehi
Oct 5, 2010·Cytokine & Growth Factor Reviews·Grant Gallagher
May 1, 2007·Expert Opinion on Therapeutic Targets·Robert SabatKerstin Wolk
Mar 31, 2012·Clinical Immunology : the Official Journal of the Clinical Immunology Society·S R HofmannC M Hedrich
Nov 16, 2010·Expert Opinion on Therapeutic Targets·Rui-Xue LengDong-Qing Ye
Jun 13, 2018·Frontiers in Immunology·Esther Caparrós, Rubén Francés

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