Promoter analysis of the chalcone synthase (chsA) gene of Petunia hybrida: a 67 bp promoter region directs flower-specific expression

Plant Molecular Biology
I M van der MeerA R Stuitje

Abstract

In order to scan the 5' flanking region of the chalcone synthase (chs A) gene for regulatory sequences involved in directing flower-specific and UV-inducible expression, a chimaeric gene was constructed containing the chs A promoter of Petunia hybrida (V30), the chloramphenicol acetyl transferase (cat) structural sequence as a reporter gene and the chs A terminator region of Petunia hybrida (V30). This chimaeric gene and 5' end deletions thereof were introduced into Petunia plants with the help of Ti plasmid-derived plant vectors and CAT activity was measured. A 220 bp chs A promoter fragment contains cis-acting elements conferring flower-specific and UV-inducible expression. A promoter fragment from -67 to +1, although at a low level, was still able to direct flower-specific expression but could not drive UV-inducible expression in transgenic Petunia seedlings. Molecular analysis of binding of flower nuclear proteins to chs A promoter fragments by gel retardation assays showed strong specific binding to the sequences from -142 to +81. Promoter sequence comparison of chs genes from other plant species, combined with the deletion analysis and gel retardation assays, strongly suggests the involvement of the TACPyAT repeats (-59 a...Continue Reading

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Citations

Jun 1, 1991·Plant Molecular Biology·W RohdeD Becker
Mar 31, 2010·PloS One·Kei-ichiro MishibaMasahiro Nishihara
Feb 1, 1992·Molecular & General Genetics : MGG·C M RommensJ Hille
Feb 2, 2016·International Journal of Molecular Sciences·Yahui HanHaiyang Jiang
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Feb 11, 2015·American Journal of Botany·Rachel A CoburnStacey D Smith
Sep 13, 2011·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·T T H DaoR Verpoorte
Feb 2, 2020·Planta·Marcos Fernando BassoMaria Fatima Grossi-de-Sa
Sep 25, 2018·Plant Biotechnology·Mirai AzumaKatsuhiro Shiratake
Nov 30, 2019·International Journal of Molecular Sciences·Yan LiShengyi Liu
Nov 30, 2019·Planta·Shahzad A PandithManzoor A Shah
Jan 1, 2014·Horticulture Research·Manjul DuttJude W Grosser

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