Promoter analysis of tumor suppressor gene PTEN: identification of minimum promoter region

Biochemical and Biophysical Research Communications
Xiaoyang ShengW K Alfred Yung

Abstract

Mutations of PTEN, a tumor suppressor gene located on chromosome 10, which encodes a protein-tyrosine and lipid-phosphatase, are prevalent in various human cancers, including glioblastoma. Despite extensive characterization of PTEN mutations in human cancers and a relatively good understanding of the molecular roles of PTEN in the control of cellular processes, little is known about modes of PTEN regulation. To understand the regulation of expression of the tumor suppressor gene PTEN, we isolated a 2212 bp fragment from the human BAC clone 46B12 DNA. The 3' end of this fragment starts at the Not I site of -745 relative to the first translation codon ATG (+1) and ends at the Sal I site of -2957 at the 5' end. Using classical 5'RACE and primer extension techniques, nine start sites were observed between -817 and -984 upstream of the ATG start site. We located a 137 bp fragment (-958/-821) as the minimum promoter region using promoter deletion and luciferase assays. A 704 bp fragment (-33/-737) downstream of the 2212 bp fragment was also cloned. As indicated by luciferase assays, the data show that this region possesses no promoter function. Interestingly, a p53 binding sequence is located within the 599 bp fragment (-1344/-745), ...Continue Reading

Citations

Dec 17, 2011·Breast Cancer Research : BCR·Tuomas HeikkinenHeli Nevanlinna
Aug 1, 2015·BoneKEy Reports·Kiyomi YamazakiStephen L-K Yen
Jan 29, 2009·International Journal of Cancer. Journal International Du Cancer·Veronica VellaFrancesco Frasca
Jun 17, 2014·Advances in Biological Regulation·Fred E BertrandGeorge Sigounas
Jul 30, 2005·World Journal of Gastroenterology : WJG·Dong-Zhu MaXi-Liang Zha
Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Salma KhanShikha Bose
Oct 18, 2002·Journal of Cellular Physiology·Gerald Thiel, Giuseppe Cibelli
Jul 5, 2021·Human Molecular Genetics·Dennis J GrencewiczCharis Eng
Mar 14, 2009·Differentiation; Research in Biological Diversity·Suzana S CoutoGerald R Cunha

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