Promoter and expression studies on an Arabidopsis thaliana dehydrin gene

FEBS Letters
D T RouseR W Parish

Abstract

A genomic clone of a group 2 lea/rab/dehydrin gene from Arabidopsis thaliana, Xero2/lti30, was cloned and sequenced. Promoter-GUS fusions were introduced into plants to analyse the promoter and determine expression patterns. Using root cultures, GUS expression was found to be moderately stimulated by abscisic acid (ABA), wounding, cold and dehydration. Results with an ABA-deficient mutant suggested endogenous ABA is required for these responses. Promoter deletion studies indicated multiple cis-acting elements are involved in the induction of the gene. GUS expression occurred in desiccated seeds, in all tissues of young seedlings and in roots (with the exception of the root tip), desiccated pollen grains, trichomes and the vascular tissues of leaves and stems in mature plants.

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Citations

Aug 17, 2004·Functional & Integrative Genomics·Chitra RaghavanTrevor W Stevenson
May 9, 2001·Biochimica Et Biophysica Acta·W F WolkersF A Hoekstra
Mar 6, 2008·BMC Genomics·Michaela Hundertmark, Dirk K Hincha
Jul 26, 2005·DNA Sequence : the Journal of DNA Sequencing and Mapping·Zhongxiang DengKexuan Tang
Mar 5, 2004·The Plant Journal : for Cell and Molecular Biology·Hans WeberEdward E Farmer
Dec 20, 2007·The Plant Journal : for Cell and Molecular Biology·Susanna Chung, Roger W Parish
Jul 20, 2006·Bioscience Reports·Zhongxiang DengKexuan Tang
Sep 8, 2011·Plant Signaling & Behavior·Moez HaninKhaled Masmoudi
Aug 11, 2000·Journal of Experimental Botany·C M VicientM Delseny
Nov 25, 2005·Plant Molecular Biology·Helen H TaiTannis Beardmore
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·G ThorlbyG Warren
Apr 27, 2017·Frontiers in Plant Science·Yuxin CaoXi Huang

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