Promoter characterization of the novel human matrix metalloproteinase-26 gene: regulation by the T-cell factor-4 implies specific expression of the gene in cancer cells of epithelial origin

The Biochemical Journal
George N MarchenkoAlex Y Strongin

Abstract

A novel matrix metalloproteinase-26 (MMP-26) is known to be specifically expressed in epithelial carcinomas. To facilitate studies of MMP-26 transcriptional regulation, we have cloned and characterized a 1 kb 5'-flanking region of the human MMP-26 gene. Altogether, our findings indicate that the MMP-26 promoter has distinctive structural and functional features among MMP genes. An unusual polyadenylation site proximal to the transcription-factor-binding sites protects transcription of the MMP-26 gene from the upstream promoters and represents a part of the stringent transcriptional regulation of the gene. The MMP-26 gene has a consensus TATA-box and one transcriptional start site located 60 and 35 nucleotides upstream of the translational start site, respectively. The MMP-26 promoter was able to drive luciferase expression in human A549 lung carcinoma, HT1080 fibrosarcoma and HEK293 embryonic kidney cells. The basal transcription efficiency of the MMP-26 promoter is relatively low, thereby explaining the minute expression of the gene in most cells and tissues. When compared with other MMP genes, the MMP-26 promoter contains binding sites for a few transcription factors. Sequential deletion and mutation analysis, and electrophor...Continue Reading

References

Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A ValsamakisJ C Alwine
Jan 1, 1996·Journal of Bioluminescence and Chemiluminescence·W W LorenzA A Szalay
Jul 27, 1999·The Journal of Biological Chemistry·H Nagase, J F Woessner
Oct 9, 1999·The American Journal of Pathology·T BrabletzT Kirchner
Mar 10, 2000·Biochimica Et Biophysica Acta·K BrewH Nagase
Jul 21, 2000·Gene·M S MuellerR Sedlacek
Nov 1, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·N Barker, H Clevers
Feb 15, 2001·Seminars in Cancer Biology·I Stamenkovic
Apr 11, 2001·Molecular Biology of the Cell·I SimchaA Ben-Ze'ev

❮ Previous
Next ❯

Citations

May 9, 2006·Cancer Metastasis Reviews·H G Munshi, M S Stack
May 9, 2006·Cancer Metastasis Reviews·Alex Y Strongin
Mar 10, 2004·The International Journal of Biochemistry & Cell Biology·Natalia D MarchenkoAlex Y Strongin
Nov 26, 2009·Oncogene·A JoannesB Nawrocki-Raby
May 2, 2009·The Journal of Biological Chemistry·Ian T StruewingCatherine D Mao
Nov 16, 2012·The Journal of Biological Chemistry·Maria V BarbolinaM Sharon Stack
Sep 24, 2005·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Toshiyuki OkuseKazushi Imai
Mar 4, 2010·Molecular Cancer Therapeutics·Elyssa M RubinBang H Hoang
Apr 6, 2006·Journal of Periodontology·Gülnur EmingilGül Atilla
Apr 9, 2005·The Journal of Investigative Dermatology·Katja AhokasUlpu Saarialho-Kere
Jul 1, 2008·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Roberta Barroso CavalcanteLélia Batista de Souza
Jun 5, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ning YangXuejun Jiang
Jul 6, 2007·Matrix Biology : Journal of the International Society for Matrix Biology·Yan ZhangPamela K Denbesten
Mar 16, 2013·Journal of Cellular and Molecular Medicine·Ravi Thakur, Durga Prasad Mishra
Jul 3, 2009·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Valéria Souza FreitasRoseana de Almeida Freitas
Feb 24, 2006·Biochemical and Biophysical Research Communications·Wolfgang HolnthonerPeter Petzelbauer
Mar 28, 2006·Biochemical and Biophysical Research Communications·Guoyi LiuCheng Zhu
Nov 24, 2004·Differentiation; Research in Biological Diversity·Alexandra SchambonyDietmar Gradl
Aug 8, 2013·Protein Expression and Purification·Tianyang MuXuexun Fang
Sep 10, 2011·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Betania Fachetti RibeiroRoseana de Almeida Freitas
Jul 24, 2003·The American Journal of Pathology·Béatrice Nawrocki-RabyPhilippe Birembaut
Mar 13, 2002·The Journal of Biological Chemistry·Natalia D MarchenkoAlex Y Strongin
Jul 26, 2002·The Journal of Biological Chemistry·Hidayatullah G MunshiM Sharon Stack
Jul 9, 2003·The Journal of Biological Chemistry·Josiane SancéauBrigitte Bauvois
Apr 23, 2003·Journal of Cancer Research and Clinical Oncology·B Lustig, J Behrens
Mar 28, 2006·The Journal of Biological Chemistry·Seokjoong KimThomas G Boyer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.