Promoter cloning in the radioresistant bacterium Deinococcus radiodurans

Journal of Bacteriology
R MeimaM E Lidstrom

Abstract

Deinococcus radiodurans is a highly radiation-resistant bacterium that is classed in a major subbranch of the bacterial domain. Since very little is known about gene expression in this bacterium, an initial study of promoters was undertaken. In order to isolate promoters and study promoter function, a series of integrative vectors for stable chromosomal insertion in D. radiodurans were developed. These vectors are based on Escherichia coli replicons that are unable to replicate autonomously in D. radiodurans and carry homologous sequences for replacement recombination in the D. radiodurans chromosome. The resulting integration vectors were used to study expression of reporter genes fused to a number of putative promoters that were amplified from the D. radiodurans R1 genome. Further analysis of these and other putative promoters was performed by Northern hybridization and primer extension experiments. In contrast to previous reports, the -10 and -35 regions of these promoters resembled the sigma(70) consensus sequence of E. coli.

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Citations

Apr 1, 2006·Applied Microbiology and Biotechnology·Alexandra D HollandMary E Lidstrom
Dec 15, 2012·Extremophiles : Life Under Extreme Conditions·Takefumi OnoderaIssay Narumi
Jul 27, 2007·Science in China. Series C, Life Sciences·GuanJun GaoYueJin Hua
Jun 12, 2009·The Journal of Biological Chemistry·Cédric A NoraisMichael M Cox
May 19, 2007·Genes & Development·Xinguo ChenSandra L Wolin
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Dea Slade, Miroslav Radman
Jan 1, 1997·Annual Review of Microbiology·J R Battista
Jan 14, 2004·BMC Microbiology·Julie Malia EggingtonMichael Matthew Cox
Sep 28, 2014·Journal of Biotechnology·Chitra Seetharam MisraShree Kumar Apte
Oct 21, 2005·Journal of Bacteriology·Marina G Kalyuzhnaya, Mary E Lidstrom
Jun 9, 2009·Plasmid·Katsuya SatohIssay Narumi
Feb 22, 2005·Molecular Microbiology·Amy K SchmidMary E Lidstrom
Dec 20, 2005·Molecular Microbiology·Edmond JolivetSuzanne Sommer
Mar 7, 2013·Molecular Microbiology·Cécile PasternakSuzanne Sommer
Sep 14, 2010·Trends in Microbiology·Bing Tian, Yuejin Hua

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