Promoter-Dependent Translation Controlled by p54nrb and hnRNPM during Myoblast Differentiation

PloS One
Nadera AinaouiA C Prats

Abstract

Fibroblast growth factor 1 (FGF1) is induced during myoblast differentiation at both transcriptional and translational levels. Here, we identify hnRNPM and p54nrb/NONO present in protein complexes bound to the FGF1 promoter and to the mRNA internal ribosome entry site (IRES). Knockdown or overexpression of these proteins indicate that they cooperate in activating IRES-dependent translation during myoblast differentiation, in a promoter-dependent manner. Importantly, mRNA transfection and promoter deletion experiments clearly demonstrate the impact of the FGF1 promoter on the activation of IRES-dependent translation via p54nrb and hnRNPM. Accordingly, knockdown of either p54 or hnRNPM also blocks endogenous FGF1 induction and myotube formation, demonstrating the physiological relevance of this mechanism and the role of these two proteins in myogenesis. Our study demonstrates the cooperative function of hnRNPM and p54nrb as regulators of IRES-dependent translation and indicates the involvement of a promoter-dependent mechanism.

References

Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·O BernardH H Reid
Feb 11, 1993·Nucleic Acids Research·R A PaysonI M Chiu
May 1, 1996·Molecular and Cellular Biology·E F MichelottiD Levens
Aug 27, 1998·The Journal of Steroid Biochemistry and Molecular Biology·R A PaysonI M Chiu
Nov 18, 2000·Journal of Molecular Biology·Y K KimS K Jang
Apr 5, 2002·Nature·Tom Maniatis, Robin Reed
Apr 2, 2005·Nature Reviews. Molecular Cell Biology·Martin Holcik, Nahum Sonenberg
Feb 8, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jung-Chun LinWoan-Yuh Tarn
Feb 9, 2007·Molecular Biology of the Cell·Stephen M LewisMartin Holcik
Jun 27, 2007·Nucleic Acids Research·Stephen D BairdMartin Holcik
Sep 4, 2007·Oncogene·S M Lewis, M Holcik
Oct 31, 2007·Molecular and Cellular Biology·Laura C CobboldAnne E Willis
Nov 13, 2007·Molecular Cell·Steve BraunsteinRobert J Schneider
Dec 18, 2007·Trends in Microbiology·Bert L Semler, Marian L Waterman
Feb 1, 2008·Development·Roshan A Jain, Elizabeth R Gavis
Apr 26, 2008·Current Opinion in Cell Biology·Shatakshi PanditXiang-Dong Fu
Jun 30, 2009·Nucleic Acids Research·Caroline ConteAnne-Catherine Prats
Oct 31, 2009·Experimental Cell Research·Marija MarkoApostolia Guialis
Mar 19, 2013·Cell·Ulrich BraunschweigBenjamin J Blencowe
Jun 12, 2013·Microvascular Research·A C PratsB Garmy-Susini

❮ Previous
Next ❯

Citations

Apr 17, 2016·Nucleic Acids Research·Gavin J KnottArcha H Fox
Nov 29, 2016·Annual Review of Genetics·Maria VeraHye Yoon Park
Dec 9, 2016·Breast Cancer Research : BCR·Elodie ChantalatFrançoise Lenfant
Jul 12, 2018·Cell Cycle·Zaur M KachaevYulii V Shidlovskii
Feb 23, 2019·International Journal of Molecular Sciences·Anne-Claire GodetAnne-Catherine Prats
Jun 10, 2016·Cancer Research·Florent MorfoisseBarbara H Garmy-Susini
Feb 26, 2021·Arteriosclerosis, Thrombosis, and Vascular Biology·Xingli Xu (徐兴丽)Cheng Zhang (张澄)
Apr 11, 2021·Journal of Cellular and Molecular Medicine·Xiuge GuFulan Wei
Jun 3, 2021·International Journal of Molecular Sciences·Guus Gijsbertus Hubert van den AkkerTim Johannes Maria Welting

❮ Previous
Next ❯

Methods Mentioned

BETA
surface plasmon resonance
transfections
transfection
PCR
chips
ChIP
immunoprecipitation
RIP
Assay
phase

Software Mentioned

Mascot Daemon
Mascot File Parsing and Quantification ( MFPaQ )
Mascot
MS
BIA

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.