PMID: 1569008May 1, 1992Paper

Promoter elements required for positive control of transcription of the Escherichia coli uhpT gene

Journal of Bacteriology
T J MerkelR J Kadner

Abstract

The uhpABCT locus of Escherichia coli encodes the transport system which allows the cell to accumulate a variety of sugar phosphates in unaltered form. The expression of uhpT, the gene encoding the transport protein, is regulated by the uhpABC gene products. The UhpA protein is required for expression; its deduced amino acid sequence shows that it belongs to a subfamily of bacterial transcription regulators including NarL, DegU, and FixJ. Members of this subfamily have an amino-terminal phosphorylation domain characteristic of so-called two-component regulators, such as OmpR, CheY, PhoB, and NtrC, and a carboxyl-terminal domain conserved among many transcriptional activators, including LuxR and MalT. The major sequence elements in the uhpT promoter that are needed for uhpT expression were investigated. Northern (RNA) hybridization analysis showed that the uhpT transcript was only present in cells induced for UhpT transport activity. The start site of transcription was identified by primer extension. Comparison of the regions upstream of the uhpT transcription start site in E. coli and Salmonella typhimurium suggested the presence of four sequence elements that might be involved in promoter function: a typical -10 region, a shor...Continue Reading

Citations

Jun 28, 2002·Molecular Microbiology·Hidehisa YoshimuraMasayuki Ohmori
Dec 12, 2001·Microbiology·Daniël T VerhammeKlaas J Hellingwerf
May 18, 2018·PloS One·Sara SandersCaren L Freel Meyers
Jan 18, 2017·The Journal of Antimicrobial Chemotherapy·M Ballestero-TéllezA Pascual
Apr 21, 2017·PLoS Pathogens·Ciaran E FinnOlivia Steele-Mortimer
Apr 28, 2001·Journal of Bacteriology·J S Wright, R J Kadner
Jun 22, 2001·Journal of Bacteriology·D L Crater, C P Moran
Jul 27, 2000·Journal of Bacteriology·Q Chen, R J Kadner
Jul 11, 2002·Journal of Bacteriology·Daniël T VerhammeKlaas J Hellingwerf
Jan 28, 2021·International Journal of Molecular Sciences·Juan Francisco Martín, Paloma Liras

Related Concepts

UhpT protein, E coli
Bacterial Proteins
DNA Sequence
Carrier Proteins
DNA Mutational Analysis
Alkalescens-Dispar Group
Hexose Transporter
Early Promoters, Genetic
Receptors, Cyclic AMP
Primary RNA Transcript

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