Promoter elements required for sugar-repression of the RAmy3D gene for alpha-amylase in rice

FEBS Letters
K ToyofukuJ Yamaguchi

Abstract

There is increasing evidence showing that cereal alpha-amylase gene expression is controlled not only by the classical hormonal regulation, but also by feed-back sugar repression. We demonstrated by in situ hybridization that the sugar repression of rice alpha-amylase gene RAmy3D takes place in scutellar epithelium cells of callus-forming rice embryos. We also used a transient expression system to study the cis-acting elements involved in the sugar repression of the RAmy3D promoter activity. Site-directed mutagenesis of the 50-bp nucleotide sequence from -172 to -123 revealed that consensus sequences of G motif (TACGTA) and TATCCA T/C motif (GATA motif as its antisense sequence) are responsible for sugar repression. The promoter sequences required for sugar repression are reported and discussed.

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Citations

Feb 19, 2000·Phytochemistry·L GuglielminettiA Alpi
Jan 15, 2002·Current Opinion in Plant Biology·Ruth R Finkelstein, Susan I Gibson
Oct 6, 1999·Current Opinion in Plant Biology·J SheenJ C Jang
Jun 22, 1999·Current Opinion in Plant Biology·T Roitsch
Jun 23, 2004·Journal of Experimental Botany·Hangsik Moon, Ann M Callahan
Sep 24, 1999·Biological Chemistry·U Wobus, H Weber
May 6, 2003·Bioscience, Biotechnology, and Biochemistry·Shigeo OtsukiJunji Yamaguchi
Oct 5, 2014·Journal of Experimental Botany·Elizabeth CordobaPatricia León
Aug 12, 2014·Plant & Cell Physiology·Amélie RabotJosé Le Gourrierec
Aug 16, 2005·The Plant Journal : for Cell and Molecular Biology·Gustavo Javier Acevedo-HernándezLuis Rafael Herrera-Estrella
Nov 1, 2016·Molecular Genetics and Genomics : MGG·Priyanka AgarwalArun K Sharma
Nov 3, 2006·Protein Expression and Purification·Song-Jae LeeDong-Il Kim
Mar 1, 2013·International Journal of Molecular Sciences·Valeria BantiPierdomenico Perata
Oct 19, 2000·Plant & Cell Physiology·K ToyofukuJ Yamaguchi
Aug 6, 2005·Plant & Cell Physiology·Anna AksamitJan Szopa
Apr 9, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Alessandra Ferreira RibasLuiz Gonzaga Esteves Vieira
Apr 25, 2020·International Journal of Molecular Sciences·Jana Jeevan RameneniYong Pyo Lim

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