Promoter hypermethylation and histone hypoacetylation contribute to pancreatic-duodenal homeobox 1 silencing in gastric cancer

Carcinogenesis
Juan MaBenjamin C Y Wong

Abstract

The expression of pancreatic-duodenal homeobox 1 (PDX1) in gastric cancer is aberrantly reduced. The aim of this study was to elucidate the regulation of DNA methylation and histone acetylation at the promoter for PDX1 silencing in gastric cancer. PDX1 expression in response to demethylation and acetylation was detected in human gastric cancer cell lines by reverse transcription-polymerase chain reaction (PCR) and western blot. Four CpG islands within the 5'-flanking region of PDX1 gene were analyzed with their transcription activities being detected by dual luciferase assay. Promoter hypermethylation was identified in gastric cancer cell lines and cancer tissues by methylation-specific PCR or bisulfite DNA sequencing PCR analysis. Histone acetylation was determined by chromatin immunoprecipitation (ChIP) assay. Demethylation by 5'-aza-2'-deoxycytidine (5'-aza-dC) and/or acetylation by trichostatin A (TSA) restored PDX1 expression in gastric cancer cells. Hypermethylation was found in four CpG islands in six of seven cancer cell lines. However, only the distal CpG island located in the promoter fragment of PDX1, F383 (c.-2063 to -1681 nt upstream of the ATG start codon) displayed significant transcriptional activity that could ...Continue Reading

References

Jan 26, 1996·The Journal of Biological Chemistry·S SharmaM R Montminy
Dec 1, 1996·Mechanisms of Development·L I LarssonH Edlund
Jun 4, 2002·Nature Reviews. Genetics·Peter A Jones, Stephen B Baylin
Jul 19, 2002·The Journal of Biological Chemistry·Eun Ran SuhPeter G Traber
Nov 8, 2003·Gene·Marco BaruccaAdriana Canapa
Jan 1, 2004·Molecular Endocrinology·Kevin GerrishRoland Stein
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·N A C S WongW F Bodmer
Sep 21, 2004·The Journal of Pathology·Emanuela PilozziLuigi Ruco
Feb 19, 2005·Nature Reviews. Cancer·Toshikazu Ushijima
Nov 24, 2005·The Journal of Biological Chemistry·Jide WangBenjamin C Y Wong
May 31, 2006·The Journal of Thoracic and Cardiovascular Surgery·Bernard GenyFrancois Piquard
Nov 15, 2006·Gastroenterology·Matthias P A EbertCatherine Lofton-Day
Feb 27, 2007·Cell·Peter A Jones, Stephen B Baylin
Mar 7, 2007·Nature Reviews. Genetics·Manel Esteller
May 31, 2007·International Journal of Cancer. Journal International Du Cancer·MingZhou GuoJames G Herman
Sep 7, 2007·Biochemical and Biophysical Research Communications·Takeshi MiyatsukaHideaki Kaneto

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Citations

Sep 16, 2011·Helicobacter·Carlos ResendeAri Ristimäki
Dec 3, 2013·Chemistry & Biology·Yuan YuanBridget K Wagner
Nov 9, 2016·Genomics·Maria Fernanda Setúbal Destro RodriguesFabio Daumas Nunes
Aug 1, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Gang XuYanling Wu
May 18, 2017·Frontiers in Medicine·Claudia I Rivas-OrtizGonzalo Castillo-Rojas
Oct 13, 2016·World Journal of Gastroenterology : WJG·Moon Kyung JooHoon Jai Chun
May 13, 2017·International Journal of Oncology·Xiao-Qing ZengShi-Yao Chen
Jul 31, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Roberto A LlerasThomas J Belbin

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