Promoter hypermethylation of PIWI/piRNA pathway genes associated with diminished pachytene piRNA production in bovine hybrid male sterility.

Epigenetics : Official Journal of the DNA Methylation Society
G-W ZhangF Zuo

Abstract

Hybrid male sterility (HMS) is a postzygotic reproductive isolation mechanism that enforces speciation. A bovine example of HMS is the yattle (also called dzo), an interspecies hybrid of taurine cattle (Bos taurus) and yak (Bos grunniens). The molecular mechanisms underlying HMS of yattle are not well understood. Epigenetic modifications of DNA methylation and P-element induced wimpy testis (PIWI)-interacting RNA (piRNAs) are important regulators in spermatogenesis. In this study, we investigated DNA methylation patterns and piRNA expression in adult testes in hybrid infertile yattle bulls and fertile cattle and yak bulls using whole genome bisulphite-seq and small RNA-seq. Promoter hypermethylation in yattle were associated with DNA methylation involved in gamete generation, piRNA metabolic processes, spermatogenesis, and spermatid development (P < 2.6 × 10-5). Male infertility in yattle was associated with the promoter hypermethylation-associated silencing of PIWI/piRNA pathway genes including PIWIL1, DDX4, PLD6, MAEL, FKBP6, TDRD1 and TDRD5. The downstream effects of silencing these genes were diminished production of 29- to 31- nucleotide pachytene piRNAs in yattle testes. Hypermethylation events at transposable element loc...Continue Reading

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Citations

Dec 4, 2020·International Journal of Molecular Sciences·Pevindu AbeysingheMurray D Mitchell
Aug 10, 2021·Frontiers in Cell and Developmental Biology·John Charles RotondoFernanda Martini

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Methods Mentioned

BETA
RNA-seq
WGBS
sRNA-seq
dissection
PCR
electrophoresis
RNA Assay
Assay
Chips
reverse transcription PCR

Software Mentioned

GraphPad Prism
DESeq2
RepeatMasker
Bismark
Trimmomatic
Seq To Name
Bowtie
DDS
samtools
GOTERM

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