Promoter methylation of TCF21 may repress autophagy in the progression of lung cancer

Journal of Cell Communication and Signaling
Baokun ChenHao Wu

Abstract

Lung cancer is a leading cause of cancer mortality worldwide. Promoter methylation of transcription factor 21 (TCF21) was frequently observed in the early stage of non-small cell lung cancer (NSCLC). However, clinical relevance and molecular functions of TCF21 in NSCLC progression remain unclear. In this study, we analyzed the associations between TCF21 expression and clinicopathological features in 100 patients with NSCLC and revealed the underlying molecular mechanisms of TCF21 methylation on cell viability, apoptosis and invasion of H1299 cells. We found that the expression of TCF21 was significantly regulated by its methylation level in patients with NSCLC and was associated with tumor stage, metastasis and invasion. Demethylation of H1299 cells by 5-aza-2'-deoxycytine (5-Aza) demonstrated that a higher level of TCF21 expression led to remarkable decreases of cell viability and invasion ability but an increase of cell apoptosis. Accordingly, TCF21 knockdown showed converse results to high expression of TCF21. TCF21 knockdown cells exhibited significantly upregulated ATG-9, BECLIN-1, and LC3-I/II expressions but decreased p62 expression compared to wildtype cells. Inhibition of autophagy by 3-methyladenine (3-MA) elevated TC...Continue Reading

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Citations

Jul 30, 2020·Stem Cells International·Shuang Jiang, Yuchen Guo
May 18, 2020·Journal of Cell Communication and Signaling·Amir Mokhlesi, Mahmood Talkhabi
Mar 4, 2020·Molecular Cancer Research : MCR·Sharada MokkapatiColin P N Dinney
Mar 18, 2021·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C F P LotfiJ L Kremer
Jul 13, 2021·World Journal of Stem Cells·Harpreet K MandhairRamin Radpour
Aug 12, 2021·Journal of Medicinal Chemistry·Yang LiLiang Ouyang

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Methods Mentioned

BETA
xenograft
PCR
electrophoresis
transfection
Feature Extractions
X-ray
biopsies
xenografts

Software Mentioned

Agilent Feature Extractions
SPSS

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