Promoter methylation of Wnt-antagonists in polypoid and nonpolypoid colorectal adenomas

BMC Cancer
Quirinus J M VoorhamBeatriz Carvalho

Abstract

Nonpolypoid adenomas are a subgroup of colorectal adenomas that have been associated with a more aggressive clinical behaviour compared to their polypoid counterparts. A substantial proportion of nonpolypoid and polypoid adenomas lack APC mutations, APC methylation or chromosomal loss of the APC locus on chromosome 5q, suggesting the involvement of other Wnt-pathway genes. The present study investigated promoter methylation of several Wnt-pathway antagonists in both nonpolypoid and polypoid adenomas. Quantitative methylation-specific PCR (qMSP) was used to evaluate methylation of four Wnt-antagonists, SFRP2, WIF-1, DKK3 and SOX17 in 18 normal colorectal mucosa samples, 9 colorectal cancer cell lines, 18 carcinomas, 44 nonpolypoid and 44 polypoid adenomas. Results were integrated with previously obtained data on APC mutation, methylation and chromosome 5q status from the same samples. Increased methylation of all genes was found in the majority of cell lines, adenomas and carcinomas compared to normal controls. WIF-1 and DKK3 showed a significantly lower level of methylation in nonpolypoid compared to polypoid adenomas (p < 0.01). Combining both adenoma types, a positive trend between APC mutation and both WIF-1 and DKK3 methyla...Continue Reading

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Citations

Dec 3, 2014·Cellular Signalling·Jitka StancikovaVladimir Korinek
Sep 5, 2015·Journal of Applied Genetics·Aleksandra Majchrzak-CelińskaWanda Baer-Dubowska
Jun 2, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Orsolya GalambBéla Molnár
Dec 10, 2015·International Journal of Oncology·Yu-Feng XiaoShi-Ming Yang
Aug 21, 2015·American Journal of Physiology. Cell Physiology·Laura NovellasdemuntVivian S W Li
May 26, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·M Alizade NainiM Borji
Aug 7, 2020·Journal of Gastroenterology and Hepatology·Carlos A Rubio

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Methods Mentioned

BETA
FCS
PCR
methylation profiling

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