Abstract
Overexpression of cyclooxygenase (COX-2) is commonly observed in human cancers. In a murine model of metastatic breast cancer, we observed that COX-2 expression and enzyme activity were associated with enhanced tumorigenic and metastatic potential. In contrast to the high COX-2 expression in metastatic tumors, transplantation of poorly tumorigenic tumor cell lines to syngeneic mice results in less COX-2 expression and less COX-2 activity in vivo. Aberrant CpG island methylation, and subsequent silencing of the COX-2 promoter, has been observed in human cancer cell lines and in some human tumors of the gastrointestinal tract. Using bisulfite modification and a methylation-specific PCR, we examined the methylation status of the COX-2 promoter in a series of four closely-related murine mammary tumors differing in COX-2 expression and metastatic potential. We showed that line 410, which does not express COX-2 in vivo, exhibited evidence of promoter methylation. Interestingly, the metastatic counterpart of this cell (line 410.4) displayed only the unmethylated COX-2 promoter, as did two additional cell lines (lines 66.1 and 67). The methylation patterns observed in vitro were maintained when these murine mammary tumor lines were tra...Continue Reading
References
Oct 1, 1979·The British Journal of Surgery·D J LeaperA Bennett
Mar 1, 1995·Preventive Medicine·R E HarrisE L Wynder
Apr 1, 1994·Journal of Cellular Physiology·R S GilbertH R Herschman
Nov 1, 1993·Postgraduate Medical Journal·G P Morgan, J G Williams
Mar 1, 1996·Epidemiology·R E HarrisW B Farrar
Oct 28, 1998·Journal of the National Cancer Institute·M M Taketo
Nov 12, 1998·Journal of the National Cancer Institute·M M Taketo
Feb 18, 1999·The American Journal of Gastroenterology·H MurataM Hori
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·M ToyotaJ P Issa
Mar 29, 2000·Journal of Cellular Physiology·R L Momparler, V Bovenzi
Jul 31, 2001·International Journal of Cancer. Journal International Du Cancer·N KunduA M Fulton
Jan 5, 2002·International Journal of Cancer. Journal International Du Cancer·Takefumi KikuchiKohzoh Imai
May 16, 2002·Journal of the National Cancer Institute·Lanlan ShenJean-Pierre J Issa
Jul 19, 2002·International Journal of Cancer. Journal International Du Cancer·Angeliki MagklaraEleftherios P Diamandis
Nov 28, 2006·Annals of Diagnostic Pathology·Cornelia LeoLars-Christian Horn
Citations
Oct 18, 2011·Cancer Metastasis Reviews·Jocelyn ReaderAmy Fulton
Apr 16, 2005·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Jinru ShiaBeiyun Chen
Jan 1, 2006·Hereditary Cancer in Clinical Practice·David Mossman, Rodney J Scott
Aug 22, 2006·Breast Cancer Research : BCR·Rosanna Zanetti-DällenbachSerenella Eppenberger-Castori
Jan 13, 2006·Nature Chemical Biology·Bethany A JanowskiDavid R Corey
Jan 20, 2012·Kidney International·Hao BaoRujun Gong
May 7, 2015·Mediators of Inflammation·Hedi Harizi
Dec 18, 2015·BMC Cancer·Hui-juan SuWei-cheng You
Mar 14, 2007·Leukemia Research·Gordana BogdanovićStevan Popović
Mar 10, 2009·Radiation Research·Brian D PieningAmanda G Paulovich
Dec 15, 2010·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sondes Karray-ChouayekhRaja Mokdad-Gargouri
Dec 21, 2010·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·X Y MengS T Zhang
Aug 4, 2020·Frontiers in Neuroscience·Arti TyagiNitesh Kumar Poddar
Aug 21, 2020·Nutrition & Metabolism·Qiang-Sheng ZhuQian-Qian Xiao
Jan 16, 2019·International Journal of Molecular Sciences·Federica AielliNadia Rucci
Jul 2, 2019·International Journal of Molecular Medicine·Shu-Mien ChuangYung-Shun Juan
Mar 12, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jean E AbrahamPaul D P Pharoah
Jan 21, 2021·International Journal of Molecular Sciences·Keita IwasakiHemanth Noothalapati
Jan 5, 2005·Biochemical and Biophysical Research Communications·Stefan SchildknechtVolker Ullrich