Promoter of a cotton fibre MYB gene functional in trichomes of Arabidopsis and glandular trichomes of tobacco.

Journal of Experimental Botany
Xiao-Xia ShangguanXiao-Ya Chen

Abstract

Cotton fibres are unicellular seed trichomes. Our previous study suggested that the cotton R2R3 MYB transcript factor GaMYB2 is a functional homologue of the Arabidopsis trichome regulator GLABRA1 (GL1). Here, the GaMYB2 promoter activity is reported in cotton (Gossypium hirsutum), tobacco (Nicotiana tabacum), and Arabidopsis plants. A 2062 bp promoter of GaMYB2 was isolated from G. arboreum, and fused to a beta-glucuronidase (GUS) reporter gene. In cotton, the GaMYB2 promoter exhibited activities in developing fibre cells and trichomes of other aerial organs, including leaves, stems and bracts. In Arabidopsis the promoter was specific to trichomes. Different from Arabidopsis and cotton that have unicellular non-glandular simple trichomes, tobacco plants contain more than one type of trichome, including multicellular simple and glandular secreting trichomes (GSTs). Interestingly, in tobacco plants the GaMYB2 promoter directed GUS expression exclusively in glandular cells of GSTs. A series of 5'-deletions revealed that a 360 bp fragment upstream to the translation initiation codon was sufficient to drive gene expression. A putative cis-element of the T/G-box was located at -233 to -214; a yeast one-hybrid assay showed that Arabi...Continue Reading

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Citations

Mar 6, 2013·Plant Molecular Biology·Ying-Bo MaoXiao-Ya Chen
Apr 30, 2013·Plant Molecular Biology·Wen-Liang XuXue-Bao Li
Oct 1, 2009·BMC Genomics·Pieter Bas KwakZhi Min Yang
May 19, 2011·The Plant Journal : for Cell and Molecular Biology·Alex Van MoerkerckeRobert C Schuurink
Feb 26, 2016·Plant Biotechnology Journal·Xiu-Ming WuXiao-Ya Chen
Oct 21, 2016·Functional & Integrative Genomics·SongTao JiuXiaoMin Wang
Nov 22, 2014·Nature Communications·Chun-Min ShanXiao-Ya Chen
Feb 24, 2018·BMC Genomics·Bingliang LiuTianzhen Zhang
Sep 3, 2010·The Plant Journal : for Cell and Molecular Biology·Jonathan M PlettSharon Regan
Apr 13, 2017·Frontiers in Plant Science·Maheshi Dassanayake, John C Larkin
Aug 6, 2019·Frontiers in Plant Science·Pearlin Shabna NazizSupriyo Sen
Aug 7, 2012·International Journal of Food Sciences and Nutrition·Rajan Katoch, Neelam Thakur
Nov 4, 2020·Molecular Genetics and Genomics : MGG·Marina NaoumkinaChristopher B Florane
Feb 3, 2021·BMC Plant Biology·Xiaojing WangLitang Lv
Mar 7, 2021·Communications Biology·Ayaka ShimokiSeiji Takeda

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Methods Mentioned

BETA
transgenic
PCR
one-hybrid

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