PMID: 11311939Apr 20, 2001Paper

Promoter of the gene encoding the 16 kDa DNA-binding and apoptosis-inducing C1D protein

Biochimica Et Biophysica Acta
K RothbarthD Werner

Abstract

The 5' region of the gene encoding the human 16 kDa DNA-binding and apoptosis-inducing C1D protein was analysed for promoter activity. Sections of this region were cloned into a promoterless vector containing the enhanced green fluorescent protein (EGFP) as reporter gene. Expressed EGFP was estimated in transfected cells by quantitative fluorescence microscopy. The sequence between mRNA positions ATG -868 and ATG -12 results in relatively highest EGFP expression in transiently transfected human and murine cells. The upstream segment immediately adjacent to the 5' end of the most active fragment was identified as an inverted LINE-1 repeat element. Transient transfection experiments point to the presence of cis-acting repressing sequences on this LINE-1 element which reduce the transcriptional activity of the basal C1D promoter in human and murine cells by more than 95%. This result supports previous evidence suggesting that LINE-1 sequences may function as regulatory elements to control the expression of nearby genes.

References

Apr 30, 1992·Biochemical and Biophysical Research Communications·K ShibataS Shibahara
Jul 21, 1995·Journal of Molecular Biology·D B StarrD K Hawley
Dec 31, 1997·The Journal of Biological Chemistry·D P WadeR Taramelli
Feb 14, 1998·The Journal of Biological Chemistry·Z YangR Lawn
Jan 22, 1998·Mammalian Genome : Official Journal of the International Mammalian Genome Society·N F BoxR A Sturm
Mar 4, 2000·Progress in Nucleic Acid Research and Molecular Biology·A V Furano

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Citations

Feb 19, 2003·Journal of Molecular Biology·Abdel Halim SalemMark A Batzer
Dec 21, 2010·Biochemistry. Biokhimii︠a︡·N SjaksteT Sjakste
Aug 13, 2003·Journal of Neurochemistry·Karin BorgesRaymond Dingledine

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