Promoter selectivity of the Bacillus subtilis response regulator DegU, a positive regulator of the fla/che operon and sacB

BMC Microbiology
Kensuke Tsukahara, Mitsuo Ogura

Abstract

The response regulator DegU and its cognate histidine kinase DegS constitute a two-component system in the Gram-positive soil bacterium Bacillus subtilis. Unphosphorylated and phosphorylated forms of DegU are known to activate target gene transcription in B. subtilis. Although phosphorylated DegU (DegU-P) regulates more than one hundred and twenty genes, the targets of unphosphorylated DegU are unknown, except for comK. We found that the fla/che (flagella and chemotaxis) operon is positively regulated by unphosphorylated DegU. The effect was most prominent in a strain bearing the functional swrAA gene, a positive regulator of fla/che. Unphosphorylated DegU bound to two regions in the fla/che regulatory region containing an inverted repeat-like sequence that resembles the inverted repeat (IR) in the comK promoter. Mutational analysis revealed that positive regulation of fla/che by SwrAA requires DegU-binding. An analysis of the DegU-P-regulated gene sacB (levansucrase gene) by footprint and mutational analyses revealed that DegU-P bound to a direct repeat (DR) of the DegU-recognition motifs, which has been shown to be functional in vivo, while unphosphorylated DegU did not. These results strongly suggest that the arrangement of ...Continue Reading

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Citations

Jun 1, 2010·Archives of Microbiology·Mitsuo OguraTeruo Tanaka
May 4, 2011·Applied Microbiology and Biotechnology·Claudia BorgmeierFriedhelm Meinhardt
Jul 5, 2013·Molecular Genetics and Genomics : MGG·Adam IwanickiMichał Obuchowski
Apr 13, 2012·Journal of Biochemistry·Mitsuo Ogura, Kensuke Tsukahara
Sep 25, 2014·Annual Review of Genetics·Sampriti Mukherjee, Daniel B Kearns
Feb 19, 2016·Environmental Science and Pollution Research International·Alberto Hernández-EligioKaty Juárez
Jul 9, 2011·Molecular Microbiology·Yi-Huang HsuehDaniel B Kearns
Nov 19, 2011·Molecular Microbiology·Joyce E Patrick, Daniel B Kearns
Mar 26, 2016·Frontiers in Microbiology·Noël MolièreKürşad Turgay
Sep 8, 2010·Molecular Microbiology·Taryn B Kiley, Nicola R Stanley-Wall
Jan 2, 2009·Microbiology·Ewan J MurrayNicola R Stanley-Wall
Dec 5, 2008·FEMS Microbiology Reviews·Daniel LopezRoberto Kolter
Mar 14, 2013·FEMS Microbiology Reviews·Sarah B Guttenplan, Daniel B Kearns
Aug 6, 2015·PLoS Genetics·Andrew M PhillipsDaniel B Kearns
Jan 29, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Mitsuo Ogura
May 28, 2017·Applied and Environmental Microbiology·Kazuo KobayashiHirofumi Yoshikawa
Apr 20, 2019·Cell Stress & Chaperones·Shaomin Yan, Guang Wu
Nov 29, 2014·Journal of Biosciences·Monica Gupta, K Krishnamurthy Rao
Jun 15, 2021·Journal of Bacteriology·Andrew M PhillipsDaniel B Kearns

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