Promoter switching allows simultaneous transcription of LANA and K14/vGPCR of Kaposi's sarcoma-associated herpesvirus

Virology
Michelle R Staudt, Dirk P Dittmer

Abstract

Latent transcription of the latency-associated nuclear antigen (LANA/ORF73) of Kaposi's sarcoma-associated herpesvirus is driven by the LANAp-c. Complexity arises during lytic reactivation, however, as the bicistronic K14/vGPCR transcript initiates 32 bp downstream of LANAp-c in the opposite orientation. We identify an Rta/ORF50-inducible LANA promoter (LANAp-i) that is distinct from the LANAp-c. LANAp-c is unaffected by Rta/ORF50. Utilization of the second, downstream LANAp-i explains how LANA and K14/vGPCR are simultaneously transcribed during de novo infection or lytic reactivation. Transactivation of LANAp-i and K14/vGPCRp requires the C-terminal activation domain of Rta/ORF50 and is mediated by DNA-binding-dependent and -independent Rta/ORF50 mechanisms. Transcriptional profiling following viral reactivation support promoter reporter phenotypes. In sum, cis-elements within the LANAp were selected to ensure faithful expression of LANA and other genes regulated by LANAp during all stages of the KSHV lifecycle despite potential interference from K14/vGPCRp activity.

References

Aug 1, 1996·Nature·Y ChangS Mittnacht
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·W ZhongD Ganem
Nov 1, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·K V KomanduriV L Ng
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·J J RussoP S Moore
Apr 4, 1998·Nucleic Acids Research·I H GregerN J Proudfoot
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·N DupinC Boshoff
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·J ChenK Yamanishi
Apr 20, 2001·Journal of Virology·M Paulose-MurphyS Zeichner
Aug 2, 2003·Journal of Cell Science·Nigel FieldMary Collins
Apr 7, 2004·The Journal of Experimental Medicine·Ilaria GuasparriEthel Cesarman
Apr 29, 2004·Journal of Virology·Blossom DamaniaDirk P Dittmer

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Citations

Jul 10, 2009·Journal of Virology·Kwun Wah WenBlossom Damania
Apr 10, 2013·Translational Research : the Journal of Laboratory and Clinical Medicine·Arun George PaulNeelam Sharma-Walia

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