PMID: 8606177Apr 1, 1996Paper

Promoters controlling expression of the alternative nitrogenase and the molybdenum uptake system in Rhodobacter capsulatus are activated by NtrC, independent of sigma54, and repressed by molybdenum

Journal of Bacteriology
M KutscheW Klipp

Abstract

The alternative nitrogenase of Rhodobacter capsulatus is expressed only under conditions of nitrogen and molybdenum depletion. The analysis of anfA-lacZ fusions demonstrated that this dual control occurred at the level of transcription of anfA, which encodes a transcriptional activator specific for the alternative nitrogenase. The anfA promoter was found to be activated under nitrogen-limiting conditions by NtrC in a sigma54-independent manner. In addition, anfA transcription was repressed by traces of molybdenum. This molybdenum-dependent repression of anfA was released in R. capsulatus mutants carrying either lesions in the high-affinity molybdenum uptake system (modABCD) or a double deletion of mopA and mopB, two genes encoding molybdenum-pterin-binding proteins. The expression of the molybdenum transport system itself was shown to be negatively regulated by molybdenum and, unexpectedly, to be also regulated by NtrC. This finding is in line with the presence of two tandemly arranged DNA motifs located in front of the R. capsulatus mopA-modABCD operon, which are homologous to R. capsulatus NtrC binding sites. Mapping of the transcriptional initiation sites of mopA and anfA revealed promoter sequences exhibiting significant ho...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·C F Higgins
Jan 1, 1992·Analytical Biochemistry·W P Deng, J A Nickoloff
May 1, 1991·Journal of Bacteriology·P HübnerT A Bickle
Mar 2, 1991·Analytical Biochemistry·K SchneiderJ Kottmann
Feb 25, 1990·Nucleic Acids Research·J ZimmermannW Ansorge
Jun 5, 1983·Journal of Molecular Biology·D Hanahan
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Sep 1, 1995·Journal of Bacteriology·J A Maupin-FurlowK T Shanmugam
Oct 1, 1994·Journal of Bacteriology·D Foster-HartnettR G Kranz
Aug 1, 1994·Journal of Bacteriology·D Foster-Hartnett, R G Kranz

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Citations

Aug 24, 2019·MicrobiologyOpen·Lisa DemtröderBernd Masepohl
Oct 18, 2000·FEMS Microbiology Letters·P S SolomonA G McEwan
May 4, 2017·Journal of Bacteriology·Marie-Christine HoffmannBernd Masepohl
Mar 12, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Alexandra MüllerEckhard Hofmann
Oct 17, 2018·Molecular Microbiology·Lisa DemtröderBernd Masepohl
Sep 14, 2019·Metallomics : Integrated Biometal Science·Arkadiusz ZupokSilke Leimkühler
Mar 30, 2001·European Journal of Biochemistry·S SiemannA Müller
Jan 22, 2017·The FEBS Journal·Gabriela de C FernandesLuciane M P Passaglia
Sep 21, 2010·Journal of Bacteriology·Jonathan GisinBernd Masepohl
Jun 20, 1998·Journal of Molecular Biology·P J CullenR G Kranz
Jun 26, 2001·Research in Microbiology·W T SelfK T Shanmugam
Jul 3, 2021·Molecular Microbiology·Nathan P WasilkoTim I Miyashiro

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