PMID: 8970999Dec 1, 1996Paper

Promoters for synthesis of the pre-C and pregenomic mRNAs of human hepatitis B virus are genetically distinct and differentially regulated

Journal of Virology
X Yu, Janet E Mertz

Abstract

Two similar, yet functionally distinct genomic RNAs are transcribed from the DNA genome of the human hepatitis B virus. The pre-C RNAs encode the precore protein which is proteolytically processed to yield e antigen. The pregenomic RNAs encode both the nucleocapsid protein and reverse transcriptase and serve as the templates for viral DNA replication. To determine whether synthesis of these two RNAs is directed from a single or a closely spaced pair of promoters, we introduced point and insertion mutations into the basal elements of the promoter that directs their synthesis. Transcription from these mutants was examined both in cell-free transcription systems derived from hepatoma (HepG2) and nonliver (HeLa) cell lines and by transient transfection of hepatoma cell lines (Huh7 and HepG2). The data from these experiments indicated that synthesis of the pre-C and pregenomic RNAs is directed by two distinct promoters and that the basal elements of these two promoters partially overlap, yet are genetically separable, with each consisting of its own transcriptional initiator and a TATA box-like sequence situated approximately 25 to 30 bp upstream of its sites of initiation. A 15-bp insertion was found to be sufficient to physically ...Continue Reading

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Citations

Sep 5, 2008·Journal of Virology·Christophe RamièrePatrice André
Jun 16, 2010·Journal of Viral Hepatitis·M Quasdorff, U Protzer
May 4, 2016·Reviews in Medical Virology·Anna Kramvis
Dec 31, 1997·Journal of Clinical Gastroenterology·M KakoM Mayumi
Sep 13, 2002·Journal of Viral Hepatitis·Naazneen MoollaPatrick Arbuthnot
Jul 14, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Stephan Schaefer
Sep 30, 2006·World Journal of Gastroenterology : WJG·Silke F FischerStephan Schaefer
Oct 2, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W E Delaney, H C Isom
Jul 14, 2007·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Shuping Tong
Dec 19, 2007·PLoS Pathogens·Xianming YuJanet E Mertz
Jan 13, 2018·Molecular Medicine Reports·Kunlun Xiang, Bin Wang
Jan 4, 1998·Journal of Gastroenterology and Hepatology·J H Ou
Aug 27, 2005·World Journal of Gastroenterology : WJG·Yun-Qing YaoShu-Hua Guo
Dec 22, 1999·Journal of Viral Hepatitis·A Kramvis, M C Kew
Oct 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Y K ChunS S Kim
Jun 21, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Yu-Chi ChouChungming Chang
Jan 28, 1998·Journal of Virology·G FourelM A Buendia
Jan 13, 2018·Journal of Virology·Hironori NishitsujiKunitada Shimotohno
Apr 2, 2003·Molecular Therapy : the Journal of the American Society of Gene Therapy·M Gabriela KramerJesus Prieto
Nov 30, 2004·Virology·Shau-Feng ChangHans Jürgen Netter
Oct 1, 2011·Future Microbiology·Matthew S Miller, Joe S Mymryk

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