Promoting Immune Efficacy of the Oral Helicobacter pylori Vaccine by HP55/PBCA Nanoparticles against the Gastrointestinal Environment

Molecular Pharmaceutics
Hai LiuYingying Xing

Abstract

The immunogenicity of oral subunit vaccines is poor partly as a result of the harsh milieu of the gastrointestinal (GI) tract. For some pathogens that restrictedly inhabit the GI tract, a vaccine that works in situ may provide more potent protection than vaccines that operate parenterally. Yet, no appropriate delivery system is available for oral subunit vaccines. In this study, we designed HP55/poly( n-butylcyanoacrylate) (PBCA) nanoparticles (NPs) to carry Helicobacter pylori ( H. pylori) subunit vaccine CCF for oral administration in a prophylactic mice model. These NPs, which are synthesized using an interfacial polymerization method, protected the CCF antigen not only from the acidic pH in simulated gastric fluid (SGF, pH 1.2) but also from the proteolysis in simulated intestinal fluid (SIF, pH 7.4). Oral vaccination of mice with HP55/PBCA-CCF NPs promoted the production of serum antigen-specific antibodies, mucosal secretory IgA, and proinflammatory cytokines. Moreover, a Th1/Th17 response and augmented lymphocytes were found in the gastric tissue of HP55/PBCA-CCF NP-immunized mice, which might eventually limit H. pylori colonization. Collectively, these results indicate that HP55/PBCA NPs are promising carriers against t...Continue Reading

References

Oct 17, 1991·The New England Journal of Medicine·A NomuraM J Blaser
Jan 10, 1998·Journal of Pharmaceutical Sciences·C DamgéP Couvreur
Apr 9, 2001·Clinical Microbiology Reviews·P L OgraR C Welliver
Oct 14, 2004·Immunology and Cell Biology·Anita GamvrellisMagdalena Plebanski
Apr 9, 2005·Alimentary Pharmacology & Therapeutics·T-U WheeldonM Sörberg
Jul 12, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Monjed ShakwehElias Fattal
Jan 5, 2006·Applied and Environmental Microbiology·Tjibbe BosmaKees Leenhouts
Feb 13, 2007·Nanomedicine : Nanotechnology, Biology, and Medicine·Catarina Pinto ReisFrancisco Veiga
Sep 11, 2007·International Journal of Pharmaceutics·Fu ChenYuan Huang
Sep 14, 2007·Journal of Pharmaceutical Sciences·Weiling HeZhi-Rong Zhang
Nov 5, 2008·Expert Review of Vaccines·Pramod K Giri, Gopal K Khuller
Nov 26, 2009·Advanced Drug Delivery Reviews·Sandra ChadwickMansoor Amiji
Dec 17, 2009·International Journal of Pharmaceutics·Arvind K JainSuresh P Vyas
Sep 16, 2011·Helicobacter·Anne Müller, Jay V Solnick
Nov 25, 2011·International Journal of Nanomedicine·Manu SharmaDipak K Majumdar
Feb 23, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Bert DevriendtEric Cox
Oct 12, 2012·Internal and Emergency Medicine·Amedeo AmedeiMario Milco D'Elios
Dec 29, 2013·Applied Microbiology and Biotechnology·Le GuoYingying Xing
Aug 30, 2014·Helicobacter·Mario M D'Elios, Steven J Czinn
Feb 25, 2015·International Journal of Nanomedicine·Laura CabezaConsolación Melguizo
Mar 5, 2015·Journal of Drug Targeting·Shiya GaoYanyu Xiao
Jul 15, 2015·Human Vaccines & Immunotherapeutics·Asma FarhadianMohammadreza Avadi
Nov 12, 2015·World Journal of Gastroenterology : WJG·Aleksandra Sokic-MilutinovicTomica Milosavljevic
Jun 12, 2016·International Journal of Biological Macromolecules·Ping GaoXiguang Chen
Aug 18, 2016·Helicobacter·Dominique VelinMarkus Gerhard
Nov 21, 2016·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Zhoulin TanYingying Xing
Mar 14, 2017·Nature Reviews. Immunology·Oliver SoehnleinChristian Weber
Sep 12, 2017·Helicobacter·Karen RobinsonLeif Percival Andersen

❮ Previous
Next ❯

Citations

Jun 13, 2019·Journal of Cellular Physiology·Masoud KeikhaMohsen Karbalaei
Feb 8, 2020·Nanomedicine·Qianyu ZhangXuefeng Xia
Oct 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jianghua LiGuangli Yu
Sep 6, 2019·Helicobacter·Philippe Lehours, Richard L Ferrero
Sep 28, 2021·Critical Reviews in Microbiology·Cláudia SousaLuís D R Melo
Dec 3, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yuan QinMingqiang Li

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Tao JiangYun-Jaie Choi
Wei sheng yan jiu = Journal of hygiene research
Yinghua LiXiaoguang Yang
European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Zhoulin TanYingying Xing
© 2022 Meta ULC. All rights reserved