Promoting roles of the secreted frizzled-related protein 2 as a Wnt agonist in lung cancer cells

Oncology Reports
Xiaomin XiaoJun Tang

Abstract

The secreted frizzled-related protein 2 (SFRP2) plays a pivotal role in the Wnt pathway, however, it functions as an agonist or an antagonist is still controversial. We profiled SFRP2 expression in several lung cancer cell lines, and found that A549 and 95-D exhibited the lowest and the highest level of SFRP2, respectively. Then we employed the SFRP2-overexpressing plasmid and siRNA to transfect A549 and 95-D cells, respectively. Through MTT assays, we found that SFRP2 knockdown inhibited cell proliferation, and halted the 95-D cells in G1 phase of the cell cycle by downregulation of CCND1 and CDK4, indicating that SFRP2 has the ability of promoting lung cancer cell proliferation. We further checked the cell properties of migration and invasion, using wound scratch assay and Transwell assays. The data showed decreased migrated and invasive 95-D cells after SFRP2 knockdown, and the observations were the opposite in the overexpressing model, implying that SFRP2 promoted lung cancer cell invasion. Moreover, the canonical Wnt pathway was also studied through detection of β-catenin by western blotting. In the SFRP2 overexpressing model, A549 cells presented stronger expression of β-catenin compared with controls, while it was the op...Continue Reading

References

Jun 7, 2000·Lancet·P C HoffmanE E Vokes
Jun 28, 2002·Cancer Cell·Ashani T WeeraratnaJeffrey M Trent
May 31, 2003·Journal of Cell Science·Yoshiaki Kawano, Robert Kypta
Jun 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Oleg Schmidt-KittlerChristoph A Klein
Sep 12, 2003·Developmental Cell·Michael T VeemanRandall T Moon
Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·Desheng LuDennis A Carson
Jan 7, 2006·Cancer Research·Hans Clevers, Eduard Batlle
Dec 5, 2006·Oncogene·L ArceM L Waterman
Jan 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·Maria MirotsouVictor Dzau
Aug 29, 2007·Acta Pharmacologica Sinica·Yihui ShiDavid M Jablons
Jan 3, 2008·Cancer Cell·Yves HüsemannChristoph A Klein
Feb 20, 2008·British Journal of Cancer·H SuzukiT Tokino
Apr 25, 2008·Nature Reviews. Cancer·Alexandra Klaus, Walter Birchmeier
Jul 2, 2008·Journal of Gastroenterology·Hideyasu TakagiYasuhisa Shinomura
May 22, 2009·Cancer Research·Andrew CourtwrightNancy Klauber-Demore
Dec 15, 2010·Nature Reviews. Clinical Oncology·Naoko TakebeS Percy Ivy
Apr 23, 2013·Molecular Cancer Therapeutics·Emily FontenotNancy Klauber-DeMore

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Citations

Sep 6, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jennillee WallaceLena Al-Harthi
Nov 4, 2020·Cancer Metastasis Reviews·Karlijn van LoonArjan W Griffioen

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Methods Mentioned

BETA
transfection
flow cytometry
xenografts

Software Mentioned

Bio
Rad CFX Manager

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