Promotion of Bamboo Mosaic Virus Accumulation in Nicotiana benthamiana by 5'→3' Exonuclease NbXRN4

Frontiers in Microbiology
Cheng-Cheng LeeMenghsiao Meng

Abstract

Bamboo mosaic virus (BaMV) has a 6.4-kb (+) sense RNA genome with a 5' cap and a 3' poly(A) tail. ORF1 of this potexvirus encodes a 155-kDa replication protein responsible for the viral RNA replication/transcription and 5' cap formation. To learn more about the replication complex of BaMV, a protein preparation enriched in the 155-kDa replication protein was obtained from Nicotiana benthamiana by a protocol involving agroinfiltration and immunoprecipitation. Subsequent analysis by SDS-PAGE and mass spectrometry identified a handful of host proteins that may participate in the viral replication. Among them, the cytoplasmic exoribonuclease NbXRN4 particularly caught our attention. NbXRN4 has been shown to have an antiviral activity against Tomato bushy stunt virus and Tomato mosaic virus. In Arabidopsis, the enzyme could reduce RNAi- and miRNA-mediated RNA decay. This study found that downregulation of NbXRN4 greatly decreased BaMV accumulation, while overexpression of NbXRN4 resulted in an opposite effect. Mutations at the catalytically essential residues abolished the function of NbXRN4 in the increase of BaMV accumulation. Nonetheless, NbXRN4 was still able to promote BaMV accumulation in the presence of the RNA silencing supp...Continue Reading

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Citations

Oct 25, 2016·The Plant Journal : for Cell and Molecular Biology·Masayoshi HashimotoShigetou Namba
Feb 6, 2017·Proceedings of the National Academy of Sciences of the United States of America·Kiwamu HyodoTetsuro Okuno
Aug 9, 2019·Nucleic Acids Research·Chaminda D GunawardeneK Andrew White
Sep 23, 2020·Nucleic Acids Research·Marion DeclercqSylvie van der Werf
Apr 1, 2017·Frontiers in Microbiology·Ying-Ping HuangChing-Hsiu Tsai
Apr 13, 2017·Frontiers in Microbiology·Menghsiao Meng, Cheng-Cheng Lee
Dec 14, 2018·Frontiers in Plant Science·Marta Budziszewska, Aleksandra Obrępalska-Stęplowska
May 11, 2017·Frontiers in Microbiology·Chi-Ping Cheng
Feb 19, 2021·Journal of Virology·Muhammad IlyasAnne E Simon

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Methods Mentioned

BETA
two-hybrid
immunoprecipitation
PCR
transfection
in vitro transcription
electrophoresis
transgenic

Software Mentioned

Mascot
Image Station
BLASTx
tBLASTn
Multigauge

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