PMID: 1992488Feb 1, 1991

Promotion of central cholinergic and dopaminergic neuron differentiation by brain-derived neurotrophic factor but not neurotrophin 3

Proceedings of the National Academy of Sciences of the United States of America
B KnüselF Hefti

Abstract

Recombinant human brain-derived neurotrophic factor (rhBDNF) and neurotrophin 3 (rhNT-3), two recently cloned molecules closely related to nerve growth factor (NGF), were produced from human cDNA expressed in human embryonic kidney cells. The recombinant proteins were tested in cultures of dissociated fetal rat brain cells containing basal forebrain cholinergic neurons. rhBDNF stimulated the differentiation of the cholinergic neurons, similar to NGF, which is well established as a neurotrophic factor for these cells. However, rhBDNF was particularly effective during the first few days in vitro, whereas the stimulatory action of rhNGF was more pronounced later in the development of the cultures. This finding indicates the existence of different time periods of responsiveness of the cholinergic neurons to BDNF and NGF. To assess the selectivity of the effect of rhBDNF on cholinergic neurons, its actions were tested in cultures of ventral mesencephalon containing dopaminergic cells. In contrast to NGF, which does not affect central dopaminergic neurons, rhBDNF increased dopamine uptake activity. The findings suggest that BDNF stimulates survival or differentiation of other cells besides the cholinergic neurons.

References

Mar 25, 1994·Biotechnology and Bioengineering·R Bellamkonda, P Aebischer
Jul 12, 2002·Journal of Neuroscience Research·Nicole C BerchtoldCarl W Cotman
Feb 1, 1993·Journal of Neuroscience Research·W HamelK Nikolics
Apr 15, 1993·Journal of Neuroscience Research·E EscandónK Nikolics
Jan 5, 2002·Synapse·Dean E DluzenJon M Walro
Apr 29, 1998·Molecular Neurobiology·K Fukunaga, E Miyamoto
Feb 1, 1996·Molecular Neurobiology·A Chalazonitis
Aug 10, 2004·Cell and Tissue Research·Kerstin Krieglstein
Jun 26, 2009·Biomedical Microdevices·Jaewon ParkArum Han
Dec 31, 2009·Cellular and Molecular Neurobiology·I Lista, G Sorrentino
Aug 17, 2012·Current Psychiatry Reports·Olga G Berwid, Jeffrey M Halperin
Oct 1, 1995·Pharmacology, Biochemistry, and Behavior·S P JawD D Truong
Jan 1, 1994·Neuroscience and Biobehavioral Reviews·L M ManessJ E Zadina
Jan 1, 1994·Pharmacology & Therapeutics·L Iacovitti
Dec 18, 1992·Brain Research. Developmental Brain Research·D NonnerJ N Barrett
Jul 16, 1993·Brain Research. Developmental Brain Research·M DownenB H Wainer
Mar 19, 1993·Brain Research. Developmental Brain Research·T RingstedtH Persson
Aug 1, 1991·Trends in Neurosciences·E Cattaneo, R McKay

Citations

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J E Bottenstein, G H Sato
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P ErnforsH Persson
Mar 23, 1990·Science·P C MaisonpierreG D Yancopoulos
Jun 1, 1989·Neuron·Y A Barde
Jul 31, 1986·Biochemical and Biophysical Research Communications·P D Rennert, G Heinrich

Related Concepts

Embryo
Fetal Structures
Proteins, Recombinant DNA
August Rats
Neurons
Nerve Growth Factor
Brain
Recombinant Brain-Derived Growth Factors
NTF3
Brain-Derived Neurotrophic Factor Measurement

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